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PepN, the major Suc-LLVY-AMC-hydrolyzing enzyme in Escherichia coli, displays functional similarity with downstream processing enzymes in Archaea and eukarya. Implications in cytosolic protein degradation

The Journal of Biological Chemistry

Dec 17, 2002

Dilip ChanduDipankar Nandi

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Abstract

Succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin (Suc-LLVY-AMC), a fluorogenic endopeptidase substrate, is used to detect 20 S proteasomal activity from Archaea to mammals. An o-phenanthroline-sensitive Suc-LLVY-AMC hydrolyzing activity was detected in Escherichia coli although it lack...read more

Mentioned in this Paper

Casein allergenic extract
Puromycin-sensitive aminopeptidase
Protein Degradation, Regulatory
Bacterial Proteins
Caseins
Alkalescens-Dispar Group
Enzymes, antithrombotic
Peptide Hydrolases
N-succinyl-leucyl-leucyl-valyl-tyrosyl-7-amino-4-methylcoumarin
NPEPPS gene
Paper Details
References
  • References48
  • Citations13
12345
  • References48
  • Citations13
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PepN, the major Suc-LLVY-AMC-hydrolyzing enzyme in Escherichia coli, displays functional similarity with downstream processing enzymes in Archaea and eukarya. Implications in cytosolic protein degradation

The Journal of Biological Chemistry

Dec 17, 2002

Dilip ChanduDipankar Nandi

PMID: 12482750

DOI: 10.1074/jbc.m207926200

Abstract

Succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin (Suc-LLVY-AMC), a fluorogenic endopeptidase substrate, is used to detect 20 S proteasomal activity from Archaea to mammals. An o-phenanthroline-sensitive Suc-LLVY-AMC hydrolyzing activity was detected in Escherichia coli although it lack...read more

Mentioned in this Paper

Casein allergenic extract
Puromycin-sensitive aminopeptidase
Protein Degradation, Regulatory
Bacterial Proteins
Caseins
Alkalescens-Dispar Group
Enzymes, antithrombotic
Peptide Hydrolases
N-succinyl-leucyl-leucyl-valyl-tyrosyl-7-amino-4-methylcoumarin
NPEPPS gene

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Chromosomal Deletion

Chromosomal deletion includes the loss of a gene sequence of DNA. The location and the genes deleted determines the significance of this abnormality. There are many identified genetic disorders that are a result from chromosomal deletion including cri du chat and Prader-Willi syndrome. Discover the latest research on chromosomal deletions here.

Oxygenation & Hydroxylation

Oxygenation is a chemical production that introduces oxygen atom into an organic compound. Hydroxylation is a chemical process that introduces a hydroxyl group (-OH) into an organic compound. In biochemistry, hydroxylation reactions are often facilitated by enzymes called hydroxylases. Cytochrome P450 (CYP) enzymes are expressed mainly in the liver and are active in mono-oxygenation and hydroxylation of various xenobiotics, including drugs and alcohols, as well as that of endogenous compounds such as steroids, bile acids, prostaglandins, leukotrienes and biogenic amines. Here is the latest research.

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Characterization and role of Peptidase N from Salmonella enterica serovar Typhimurium

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Paper Details
References
  • References48
  • Citations13
12345
  • References48
  • Citations13
12

Download from

Publisher
PubMed
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