Pepscan mapping and fusion-related properties of the major phosphatidylserine-binding domain of the glycoprotein of viral hemorrhagic septicemia virus, a salmonid rhabdovirus

Virology
A Estepa, J M Coll

Abstract

The binding of labeled phosphatidylserine (PS) to a collection of synthetic 15-mer peptides covering full-length glycoprotein G (G) of viral hemorrhagic septicemia virus (VHSV), a salmonid rhabdovirus, showed three dominant overlapping reactive peptides. This major PS-binding region was contained in a 28-mer peptide (p2; aa 82-109) with consecutive hydrophobic amino acid a-d heptad repeats (putative amphipathic alpha-helix) and 2 carboxy-terminal arginines. This 28-mer peptide showed a 10-fold higher apparent specific activity for PS binding than the 15-mer peptides. Binding to PS was also detected with virion-purified protein G but was not detected with other viral proteins. The highest apparent specific activity for PS binding was found with purified VHSV particles by both solid-phase and liquid assays. In contrast to the pH-independent PS binding to peptide p2, binding to virions was optimal at pH 5.6. PS binding to purified VHSV was greatly reduced by protease or detergent treatments that removed protein G, by treatment at pH 7.6, or by anti-p2 mouse antibodies at pH 5.6. The PS-binding region seems to be related to viral-host cell fusion since anti-p2 mouse antibodies inhibited VHSV-infected cell to cell fusion (fusion fro...Continue Reading

Citations

Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·M R SutherlandE L Pryzdial
Sep 27, 2005·European Biophysics Journal : EBJ·Fabiana A CarneiroAndrea T Da Poian
May 12, 2004·Veterinary Immunology and Immunopathology·A RochaJ M Coll
May 20, 1999·Molecular Membrane Biology·Y GaudinR Ruigrok
Nov 7, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Carolina G SarzedasFabio C L Almeida
Jun 4, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A T Da PoianF Stauffer
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melissa K CallahanAndrew J Henderson
Aug 10, 1999·Journal of Virology·M BearzottiM Bremont

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