Pepsin, an aspartic protease, converts porcine big endothelin to 21-residue endothelin

Biochemical and Biophysical Research Communications
M TakaokaS Morimoto

Abstract

Porcine big endothelin (big ET-39) at 1 nM, a concentration with no influence on contractile activity in isolated rat aorta, induced a slow-onset and sustained contraction by the pre-incubation with pepsin. When the incubation mixture of big ET-39 with pepsin was analyzed by high-performance liquid chromatography on an octadecyl silica column, two major products of pepsin hydrolysis were obtained; their amino acid sequences were identical with those of 21-residue endothelin (ET-21) and a C-terminal peptide of big ET-39, big ET (22-39), respectively. On the other hand, no degradation of ET-21 was observed by pepsin treatment. These results indicate that pepsin specifically cleaves a Trp21-Val22 bond in the big ET-39 molecule, producing ET-21 and big ET (22-39). Thus, the possibility that pepsin-like aspartic protease may participate in the conversion of big ET-39 to ET-21 in vivo warrants further attention.

References

Apr 28, 1989·Biochemical and Biophysical Research Communications·Y MatsumuraS Morimoto
Jun 15, 1989·Biochemical and Biophysical Research Communications·E G McMahonA J Trapani
Jul 14, 1989·Biochemical and Biophysical Research Communications·T EmoriF Marumo
Nov 15, 1988·Biochemical and Biophysical Research Communications·S KimuraT Masaki

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Citations

Mar 1, 1995·FEMS Immunology and Medical Microbiology·H Tsushima, H Mine
Jul 13, 2001·Pharmacology & Therapeutics·S Naicker, K D Bhoola
Jan 1, 1996·Pharmacology & Therapeutics·G A Gray, D J Webb
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E G McMahonM K Stern
Sep 1, 1992·Pulmonary Pharmacology·F PonsP Braquet
Oct 29, 1990·FEBS Letters·W E LeesJ Kay
Oct 10, 1998·Neurosurgery·M Zimmermann, V Seifert
Sep 1, 1993·International Journal of Peptide and Protein Research·P SavageA Y Jeng
Jun 29, 1990·Biochemical and Biophysical Research Communications·T SawamuraT Masaki
Jan 26, 1996·Biochemical Pharmacology·A J Turner, L J Murphy
Dec 31, 1990·Biochemical and Biophysical Research Communications·M TakaokaS Morimoto

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