Pepsin Digest of Gliadin Forms Spontaneously Amyloid-Like Nanostructures Influencing the Expression of Selected Pro-Inflammatory, Chemoattractant, and Apoptotic Genes in Caco-2 Cells: Implications for Gluten-Related Disorders.

Molecular Nutrition & Food Research
Maria Georgina HerreraVeronica I Dodero

Abstract

Proteolysis-resistant gliadin peptides are intensely investigated in biomedical research relates to celiac disease and gluten-related disorders. Herein, the first integrated supramolecular investigation of pepsin-digested gliadin peptides (p-gliadin) is presented in combination with its functional behavior in the Caco-2 cell line. First, gliadins are degraded by pepsin at pH 3, and the physicochemical properties of p-gliadin are compared with gliadin. An integrated approach using interfacial, spectroscopic, and microscopic techniques reveals that the p-gliadin forms spontaneously soluble large supramolecular structures, mainly oligomers and fibrils, capable of binding amyloid-sensitive dyes. The self-assembly of p-gliadin starts at a concentration of 0.40 µg mL-1 . Second, the stimulation of Caco-2 cells with the p-gliadin supramolecular system is performed, and the mRNA expression levels of a panel of genes are tested. The experiments show that p-gliadin composed of supramolecular structures triggers significant mRNA up-regulation (p < 0.05) of pro-apoptotic biomarkers (ratio Bcl2/Bak-1), chemokines (CCL2, CCL3, CCL4, CCL5, CXCL8), and the chemokine receptor CXCR3. This work demonstrates that p-gliadin is interfacial active, f...Continue Reading

References

Nov 1, 1968·Biochemistry·D D KasardaW Gaffield
Nov 5, 2003·Journal of Cellular and Molecular Medicine·Alexandrina Burlacu
Jun 8, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ronit SneerHanna Rapaport
Jun 1, 2005·Microbes and Infection·Veerle SnoeckEric Cox
Sep 24, 2005·Nano Letters·Andrea LomanderShuguang Zhang
Oct 13, 2006·The Journal of Physical Chemistry. B·Ning DuChoy L Hew
Jun 3, 2008·Carbohydrate Research·Naghmeh SattarahmadyNader Sheibani
Apr 20, 2010·Biochimica Et Biophysica Acta·Matthew Biancalana, Shohei Koide
Jan 26, 2011·Journal of Agricultural and Food Chemistry·Bert G ThewissenJan A Delcour
Feb 26, 2011·Current Protein & Peptide Science·Carlos W Bertoncini, M Soledad Celej
Sep 8, 2012·Nanoscale·Silvia MarchesanPatrick G Hartley
Aug 20, 2015·Critical Reviews in Food Science and Nutrition·Alessandra BordoniGuy Vergères
Sep 17, 2015·Soft Matter·M G HerreraV I Dodero
Nov 6, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Silvia MarchesanKatie E Styan
Feb 22, 2016·Colloids and Surfaces. B, Biointerfaces·María G HerreraVerónica I Dodero
May 1, 2016·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Antonio Di SabatinoGino Roberto Corazza
Dec 8, 2017·Biophysics Reviews·Eri Chatani, Naoki Yamamoto
Mar 12, 2018·Colloids and Surfaces. B, Biointerfaces·M G HerreraV I Dodero
Mar 14, 2018·ChemistryOpen·Karen M LammersVeronica I Dodero
Mar 20, 2018·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Prashant SinghGovind K Makharia
Apr 25, 2018·Nanomedicine : Nanotechnology, Biology, and Medicine·M G HerreraV I Dodero
Apr 29, 2018·Molecular Nutrition & Food Research·Charlene B Van BuitenRyan J Elias
May 28, 2019·Physical Chemistry Chemical Physics : PCCP·María Alejandra SequeiraVerónica Isabel Dodero
Jul 5, 2019·International Journal of Molecular Sciences·Paola CicatielloPaola Giardina
Jul 25, 2019·BMC Medicine·Giacomo CaioAlessio Fasano
Jul 25, 2019·Trends in Molecular Medicine·Asbjørn ChristophersenLudvig M Sollid
Oct 8, 2019·Physical Chemistry Chemical Physics : PCCP·María Julia AmundarainVerónica I Dodero
Nov 9, 2019·Chembiochem : a European Journal of Chemical Biology·Nicolo TonaliNorbert Sewald
Nov 22, 2019·Frontiers in Nutrition·Gloria SerenaAlessio Fasano
Jan 14, 2020·Advances in Protein Chemistry and Structural Biology·John A Carver, Carl Holt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Acta Paediatrica Academiae Scientiarum Hungaricae
M SzabolcsB Szabó
Annales paediatrici. International review of pediatrics
E FREUDENBERG, M JUST
Clinica Chimica Acta; International Journal of Clinical Chemistry
G Bruce, J F Woodley
Acta Paediatrica Academiae Scientiarum Hungaricae
S CsorbaB Szabó
Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
Erika MonguzziLeda Roncoroni
© 2021 Meta ULC. All rights reserved