PMID: 3320889Oct 1, 1987Paper

Peptidase in the plasma of mice infected with Trypanosoma brucei brucei

Parasitology
G KnowlesD D Whitelaw

Abstract

The plasma of mice infected with pleomorphic Trypanosoma brucei brucei contains a peptidase which has the same electrophoretic mobility on starch gels as a parasite peptidase. An enzyme with this electrophoretic mobility was not detected in the plasma of uninfected mice. The molecular weight of this enzyme in either parasite lysate or plasma from infected mice was approximately 40,000 Da when assayed on a size exclusion column using high-performance liquid chromatography. The enzyme can cleave the dipeptides leu-ala, val-leu and pro-leu, but not the dipeptide phe-ala. The enzyme also cleaved the tripeptides tyr-tyr-tyr and leu-gly-gly. Another parasite peptidase which migrates on starch gels to a different position than the above-mentioned peptidase cleaved the dipeptides leu-ala, val-leu and pro-leu but could not cleave the tripeptides tyr-tyr-tyr or leu-gly-gly. Furthermore, incubation of this parasite peptidase with normal mouse plasma at 37 degrees C resulted in an apparent loss of detectable activity. It is postulated that the plasma of mice modifies either the charge or enzymic activity of this peptidase. We speculate that the parasite peptidase present in the plasma of mice infected with T. brucei could contribute to pat...Continue Reading

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Citations

Apr 1, 1989·Molecular and Biochemical Parasitology·G KnowlesS J Black
Apr 1, 1993·Comparative Biochemistry and Physiology. Comparative Physiology·B SoudanD Tetaert
Jan 27, 2009·Infection and Immunity·Nicolas Antoine-MoussiauxDaniel Desmecht
Nov 30, 2011·Molecular and Biochemical Parasitology·Richard T KangetheTheresa H T Coetzer
Oct 8, 2008·Immunological Reviews·Henry TabelMeiqing Shi
Jun 14, 2016·Pathology Research International·Alpidio A Boada-SucreArmando Reyna-Bello
Oct 20, 2019·Biochimie·Flávia Nader MottaIzabela Marques Dourado Bastos

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