PMID: 2503589Sep 1, 1989Paper

Peptidasic activities associated with acetylcholinesterase are due to contaminating enzymes

Journal of Neurochemistry
F Checler, J P Vincent

Abstract

The esterasic and peptidasic activities of two different sources of acetylcholinesterase purified from electric eel were examined. Hydrolyses of leucine-enkephalin and neurotensin indicated that both sources exhibited exopeptidasic and tryptic-like activities. However, the enzyme preparation which appeared 10-fold enriched with regard to the esterasic activity was found to display a 50- and 185-fold lower tryptic-like and exopeptidasic function, respectively. This lack of parallelism in the enrichment of the various activities seemed to indicate that they were not co-purified. Immunoprecipitation experiments performed with monoclonal antibodies directed towards the catalytic subunit of globular or asymmetric forms of electric eel acetylcholinesterase allowed the physical dissociation of esterasic and peptidasic functions and therefore confirmed that the ability of acetylcholinesterase to hydrolyze various neuropeptides was likely due to contaminating peptidases.

References

Sep 15, 1976·European Journal of Biochemistry·J Massoulié, S Bon
Feb 11, 1987·Journal of Immunological Methods·M M McKinney, A Parkinson
Jan 1, 1980·Neuroscience·I W ChubbG H White
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Citations

May 1, 1992·Annals of Neurology·M MesulamC Geula
May 1, 1996·International Journal for Parasitology·D L Lee
Jan 1, 1990·Life Sciences·R LeducC Lazure
Nov 19, 1993·Brain Research. Developmental Brain Research·C GeulaC C Kuo
Dec 1, 1992·Trends in Neurosciences·M E Appleyard
Jul 1, 1993·Progress in Neurobiology·J MassouliéF M Vallette
Aug 1, 1992·Neuroscience·H Winkler, R Fischer-Colbrie
Jul 16, 2003·Peptides·Paolo TucciPacifico Valeri
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C I WrightM M Mesulam
Jan 1, 1995·Progress in Histochemistry and Cytochemistry·P G Layer, E Willbold
Mar 31, 1998·Protein Expression and Purification·S Estrada-Mondaca, D Fournier

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