PMID: 8458691Feb 1, 1993Paper

Peptide amidation by enzymatic transacylation and photolysis

International Journal of Peptide and Protein Research
D B HenriksenO Buchardt

Abstract

A series of model peptides with a C-terminal protected amide group were prepared by enzymatic transacylation. The protection groups were removed by photolysis to give the warranted peptide amides in high yields. Furthermore, fragments of human calcitonin were prepared. Various protective groups were employed, and the pH, solvent and concentration dependency of the enzymatic transcylation were examined. The photo-cleavage reaction was examined for wavelength, concentration and pH dependency. It was shown that the optimal yields required addition of a chemical scavenger for the photolysis byproducts.

References

Feb 1, 1991·International Journal of Peptide and Protein Research·K BreddamM Meldal
Sep 1, 1990·International Journal of Peptide and Protein Research·D S KingG B Fields
Jun 1, 1990·International Journal of Peptide and Protein Research·N Kneib-CordonierG Barany
Sep 1, 1989·International Journal of Peptide and Protein Research·W StüberG Breipohl
Jan 1, 1988·Annual Review of Physiology·B A Eipper, R E Mains

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Citations

Apr 4, 2019·Biomaterials Science·Jingyan ZhangMingdi Yang
Apr 1, 1995·International Journal of Peptide and Protein Research·M W DaveyJ Vandekerckhove
Jul 1, 1997·European Journal of Biochemistry·V CerovskýJ Brass
Jun 24, 2000·The Journal of Organic Chemistry·C P SalernoA P Patron

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