PMID: 11931583Apr 5, 2002Paper

Peptide based vaccine design: synthesis and immunological characterization of branched polypeptide conjugates comprising the 276-284 immunodominant epitope of HSV-1 glycoprotein D

Journal of Peptide Science : an Official Publication of the European Peptide Society
Gábor MezoFerenc Hudecz

Abstract

The importance of the length and conjugation site of a protective epitope peptide (276SALLEDPVG284) from glycoprotein D of herpes simplex virus in branched polypeptide conjugates has been investigated. A new set of peptides, with a single attachment site and truncated sequences, was prepared. The immunogenicity of conjugates and the specificity of antibody responses elicited were investigated in BALB/c, C57/B1/6 and CBA mice. It was found that the covalent coupling of the peptide comprising the 276-284 sequence of gD through its Asp residue at position 281 did not influence the immunogenic properties of the epitope, while involvement of the side chain of Glu at position 280 almost completely abolished immunogenicity. These results clearly indicated that the conjugation site of the epitope peptide influenced the intensity and specificity of antibody responses. Comparison of the immunological properties of conjugates containing truncated gD peptides revealed the presence of two epitopes within the 276-284 region. One of the proposed epitopes is situated at the N-terminal (276-281) region, while the other is located at the C-terminal end of the sequence (279-284). Binding data demonstrated that some of the peptides comprising thes...Continue Reading

References

Apr 1, 1991·FEMS Microbiology Immunology·S Welling-WebsterG W Welling
Jan 1, 1987·Annual Review of Microbiology·B Roizman, A E Sears
Aug 1, 1997·Current Opinion in Biotechnology·T Ben-Yedidia, R Arnon
Feb 10, 2000·The Journal of Peptide Research : Official Journal of the American Peptide Society·G MezoF Hudecz
May 16, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·E DrakopoulouF Hudecz
Aug 21, 2001·Molecular Cell·A CarfíD C Wiley

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Citations

Jun 18, 2016·International Journal of Genomics·Vikas SharmaTulika Prakash
Jan 7, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Gábor MezöFerenc Hudecz
May 3, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Regina TugyiFerenc Hudecz
May 8, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·András KolozsiBéla Gyurcsik

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