Peptide-binding motif prediction by using phage display library for SasaUBA*0301, a resistance haplotype of MHC class I molecule from Atlantic Salmon (Salmo salar)

Molecular Immunology
Heng ZhaoHuub F J Savelkoul

Abstract

The structure of the peptide-binding specificity of major histocompatibility complex (MHC) class I has been analyzed extensively in human and mouse. For fish, there are no crystallographic models of MHC molecules, neither are there data on the peptide-binding specificity. In this study, we describe for the first time the identification of a fish class I peptide-MHC ligand binding motif. Phage display technology using both 7 mer and 12 mer libraries enabled us to identify peptide ligands with unique specificity that interacts with the recombinant Salmon MHC class I molecule. The recombinant proteins, beta 2m/SasaUBA*0301, were produced in Escherichia coli, in which the carboxyl terminus of beta 2-microglobulin is joined together with a flexible (GGGGS)3 linker to the amino terminus of the heavy chain. One hundred and seven individual phages bound to beta 2m/SasaUBA*0301 were isolated after four rounds of panning from the 7 mer random-peptide library. The peptide encoding sequences were determined and peptide alignment led to the prediction of position-specific anchor residue. A prominent proline at position 2 was observed and we predict that it might be one of the anchors at the N-terminus. Meanwhile, phage display peptide libra...Continue Reading

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Citations

Oct 12, 2010·Journal of Computer-aided Molecular Design·Constanza CárdenasF Javier Luque
Feb 6, 2014·Cellular and Molecular Life Sciences : CMLS·Peter OverathHans-Georg Rammensee
Apr 17, 2012·Developmental and Comparative Immunology·Lv-yun ZhuJian-zhong Shao
Oct 11, 2012·Immunological Reviews·Michael E BirnbaumK Christopher Garcia
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka

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