Peptide Bond Ultraviolet Absorption Enables Vibrational Cold-Ion Spectroscopy of Nonaromatic Peptides

The Journal of Physical Chemistry Letters
Aleksandr Y PereverzevOleg V Boyarkin

Abstract

Peptide-bond VUV absorption is inherent to all proteins and peptides. Although widely exploited in top-down proteomics for photodissociation, this absorption has never been spectroscopically characterized in the gas phase. We have measured VUV/UV photofragmentation spectrum of a single peptide bond in a cryogenically cold protonated dipeptide. Although the spectrum appears to be very broadband and structureless, vibrational pre-excitation of this and even larger cold peptides significantly increases the UV dissociation yield for some of their photofragments. We use this effect to extend the technique of IR-UV photofragmentation vibrational spectroscopy, developed for aromatic peptides, to nonaromatic ones and demonstrate measurements of conformation-specific and nonspecific IR spectra for di- to hexa-peptides.

References

May 30, 2002·Analytical Chemistry·Anne E Counterman, David E Clemmer
Dec 19, 2002·Biophysical Chemistry·Robert W Woody, Axel Koslowski
Aug 29, 2006·Journal of the American Society for Mass Spectrometry·Kyung Mi ChoiMyung Soo Kim
Dec 1, 2007·The Journal of Physical Chemistry. a·V Alvin Shubert, Timothy S Zwier
Feb 26, 2009·Mass Spectrometry Reviews·James P Reilly
Mar 6, 2010·Journal of the American Chemical Society·Natalia S NagornovaOleg V Boyarkin
Jun 25, 2010·The Journal of Physical Chemistry. a·Woon Yong Sohn, Jae Shin Lee
Aug 27, 2013·Accounts of Chemical Research·Arron B WolkMark A Johnson
Jan 1, 1993·Journal of the American Society for Mass Spectrometry·L L Griffin, D J McAdoo
Apr 3, 2014·The Review of Scientific Instruments·Oleg V Boyarkin, Vladimir Kopysov
Oct 24, 2014·The Journal of Chemical Physics·Aleksandra V ZabugaThomas R Rizzo
May 3, 2012·The Journal of Physical Chemistry Letters·Christopher M LeavittMark A Johnson
Sep 17, 2015·The Journal of Chemical Physics·Antoine MassonThomas R Rizzo
Jun 29, 2016·The Journal of Physical Chemistry. a·Aleksandr Y PereverzevOleg V Boyarkin
Jan 21, 2017·Physical Chemistry Chemical Physics : PCCP·A Y Pereverzev, O V Boyarkin
Oct 19, 2017·Angewandte Chemie·Aleksandr Y PereverzevOleg V Boyarkin

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Citations

Dec 18, 2019·The Journal of Physical Chemistry Letters·Aleksandr Y PereverzevOleg V Boyarkin

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