Abstract
Recently, obesity has increased due to a variety of reasons, including the availability of 'fast food' and high-fat diets. Developing anti-obesity functional drugs and foods from natural sources may offer solutions to this global concern. Generally, tuna is a high-protein, low-fat and low-calorie food with various bioactive effects. It may improve memory, reduce cholesterol levels and positively affect the development of brain cells. In this study, we screened the anti-obesity potential of peptides derived from tuna protein. We then observed protein bands by the Coomassie blue staining of a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel. The protein mixture was concentrated and desalted using in-gel trypsin digestion and a C18 nano column and Poros R2 reversed-phase preparation, prior to quadrupole time-of-flight mass spectrometry (Q-TOF MS/MS). We screened the peptides for their ability to affect adipogenesis in 3T3-L1 adipocytes. We also measured glucose uptake, triglyceride levels and lipid droplets using Oil Red O staining. As a result, we confirmed that one peptide inhibited adipocyte differentiation. We also observed the expression of obesity-related genes by western blot analysis and reverse tra...Continue Reading
References
May 1, 1975·Cell·H Green, O Kehinde
Jan 1, 1987·The American Journal of Cardiology·J MehtaT Wargovich
Dec 1, 1985·Annals of Internal Medicine·E A Lew
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·E HuB M Spiegelman
Aug 1, 1995·The Biochemical Journal·C M Smas, H S Sul
Jan 1, 1994·Annual Review of Nutrition·P CorneliusM D Lane
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Dec 1, 1996·Current Opinion in Cell Biology·R P BrunB M Spiegelman
Nov 7, 1998·The Journal of Biological Chemistry·G J DarlingtonO A MacDougald
Apr 15, 2000·Nature·P G Kopelman
Mar 29, 2001·Diabetes·R H Unger, Y T Zhou
Mar 29, 2001·Annual Review of Public Health·T L Visscher, J C Seidell
Oct 19, 2004·Life Sciences·Taeil JeonDong Ki Park
Feb 11, 2005·Nutrition Journal·Ravi Kant
Oct 3, 2006·Cell Metabolism·Stephen R Farmer
Apr 2, 2010·American Journal of Physiology. Cell Physiology·Jiyun AhnTaeyoul Ha
Jul 21, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Stephen D Hursting, Marcie J Hursting
Jan 16, 2013·The Journal of Nutritional Biochemistry·Haeyong LeeYoosik Yoon
Apr 16, 2013·Biochemical and Biophysical Research Communications·Dong Min KimWon Kon Kim
Aug 8, 2013·PloS One·Yonghan HeChanghao Sun
Citations
Nov 18, 2015·International Journal of Molecular Medicine·Young-Min KimTaek-Jeong Nam
Aug 9, 2016·International Journal of Molecular Medicine·Youngmin KimTaek-Jeong Nam
Sep 29, 2018·Food Science and Biotechnology·Min-Ji KimDong-Hyun Ahn
Mar 6, 2019·Frontiers in Nutrition·Maushmi S Kumar
Aug 11, 2018·Genes & Genomics·Han-Heom Na, Keun-Cheol Kim
Jun 6, 2015·International Journal of Molecular Medicine·Young-Min KimTaek-Jeong Nam
Jan 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Anna IwaniakPiotr Minkiewicz
Mar 4, 2021·Marine Drugs·Xing-Wei XiangBin Zheng