Peptide fingerprinting of snake venoms by direct infusion nano-electrospray ionization mass spectrometry: potential use in venom identification and taxonomy

Journal of Mass Spectrometry : JMS
Gustavo H M F SouzaStephen Hyslop

Abstract

Fingerprinting by mass spectrometry has been increasingly used to study venom variations and for taxonomic analyses based on venom components. Most of these studies have concentrated on components heavier than 3 kDa, but Bothrops snake venoms contain many biologically active peptides, principally C-type natriuretic peptides and bradykinin-potentiating peptides (BPPs). In this work, we have examined the peptide profile of Bothrops venoms (B. alternatus, B. erythromelas, B. insularis, B. jararaca, B. jararacussu, B. leucurus and B. moojeni) using direct infusion nano-electrospray ionization mass spectrometry (nano-ESI-MS) subjecting the data further to principal components analysis (PCA) to assess whether the peptide distributions are reliable in distinguishing the venoms. ESI-MS of a low molar mass fraction obtained by ultrafiltration of each venom (5 kDa nominal cutoff filters) revealed that the venoms have a variety of peptides in common but that each venom also contains taxonomic marker peptides not shared with other venoms. One BPP peptide, QGGWPRPGPEIPP, was found to be common to the seven Bothrops species examined. This peptide may represent a specific marker for this genus since it was not found in the venom of the South ...Continue Reading

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Citations

Aug 8, 2012·Molecular & Cellular Proteomics : MCP·Alexandre K TashimaSolange M T Serrano
Feb 14, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Sylvia Sanches CortezziEdson Borges
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Oct 23, 2020·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Adriana SimizoAlexandre K Tashima
Sep 25, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tarek Mohamed Abd El-AzizJames D Stockand

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