Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood-brain barrier in rats

International Journal of Nanomedicine
Salvatore ValianteStefania Galdiero

Abstract

Peptide gH625, derived from glycoprotein H of herpes simplex virus type 1, can enter cells efficiently and deliver a cargo. Nanoparticles armed with gH625 are able to cross an in vitro model of the blood-brain barrier (BBB). In the present study, in vitro experiments were performed to investigate whether gH625 can enter and accumulate in neuron and astrocyte cell lines. The ability of gH625 to cross the BBB in vivo was also evaluated. gH625 was administered in vivo to rats and its presence in the liver and in the brain was detected. Within 3.5 hours of intravenous administration, gH625 can be found beyond the BBB in proximity to cell neurites. gH625 has no toxic effects in vivo, since it does not affect the maximal oxidative capacity of the brain or the mitochondrial respiration rate. Our data suggest that gH625, with its ability to cross the BBB, represents a novel nanocarrier system for drug delivery to the central nervous system. These results open up new possibilities for direct delivery of drugs into patients in the field of theranostics and might address the treatment of several human diseases.

Citations

Dec 25, 2019·Antimicrobial Agents and Chemotherapy·Alaa RiezkSimon L Croft
Jun 27, 2019·Scientific Reports·Giuseppina IachettaSalvatore Valiante
Apr 3, 2020·Scientific Reports·Emilia GaldieroStefania Galdiero
Aug 27, 2017·Scientific Reports·Annarita FalangaStefania Galdiero
Nov 21, 2018·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Hui LiuWei Lu
Feb 3, 2021·Neurochemical Research·Hung NguyenSelva Baltan
Aug 13, 2021·Frontiers in Cellular Neuroscience·Aaron J MoulsonPeggy Assinck

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

Zen
ImageJ

Related Concepts

Related Feeds

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.