PMID: 6166029Jan 1, 1981Paper

Peptide immunocytochemistry

Progress in Histochemistry and Cytochemistry
L I Larsson

Abstract

The potentials and limitations of immunocytochemical techniques are discussed with special reference to the light and electron microscopical localization of secretory peptides. Particular attention is given to methods for preserving peptide antigenicity and tissue structure as well as to control procedures for establishing immunocytochemical specificity. Additionally, comparisons between existing immunocytochemical methods with respect to their sensitivity, reliability and specificity are made. It is emphasized that peptide antigens differ with respect to physicochemical properties and that therefore different antigenic regions, even of the same peptide chain, are variously affected by different fixation and pretreatment conditions. The meaning of immunocytochemical specificity is extensively discussed. It is emphasized that adverse tissue pretreatment conditions make specific immunocytochemistry impossible. The need for concerted use of staining controls and specificity (absorption) controls is underlined. Additionally, antibodies must be regarded as region- or site-specific detection reagents, rather than as truly peptide-specific reagents. Thus, experiments employing either a single antiserum or sets of antisera with the sam...Continue Reading

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