Peptide-induced HLA-E expression in human PBMCs is dependent on peptide sequence and the HLA-E genotype

Tissue Antigens
Nina LauterbachM G J Tilanus

Abstract

Human Leukocyte Antigen (HLA)-E is a low-polymorphic non-classical HLA class I molecule which plays a crucial role in immune surveillance by presentation of peptides to T and natural killer (NK) cells. HLA-E polymorphism is related to HLA-E surface expression and is associated with patient outcome after stem cell transplantation. We aim to investigate the regulation of HLA-E expression level in peripheral blood mononuclear cells (PBMCs) of healthy individuals homozygous for HLA-E*01:01 or HLA-E*01:03, by using a panel of HLA-E binding peptides derived from CMV, Hsp60 and HLA class I. Basal and peptide-induced HLA-E surface expression levels were higher in PBMC from HLA-E*01:03 homozygous subjects as compared to PBMC from HLA-E*01:01 homozygous subjects. HLA-E mRNA levels were comparable between the two genotypes and remained constant after peptide stimulation. HLA-E surface expression seemed to be not only dependent on the HLA-E genotype, but also on the sequence of the peptide as evidenced by the profound difference in HLA-E upregulation with the Hsp60 and the B7 peptide. Our results showed that peptide-induced HLA-E expression is regulated at the posttranscriptional level as extracellular peptide stimulation did not influence...Continue Reading

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Citations

Aug 6, 2016·Journal of Immunology Research·Julie Di CristofaroChristophe Picard
Jan 28, 2017·HLA : Immune Response Genetics·T I OlieslagersM G J Tilanus
Apr 11, 2019·Clinical and Experimental Immunology·H R SharpeT Lambe
Sep 3, 2016·Oncotarget·Barbara SeligerArndt Hartmann
Feb 6, 2020·Frontiers in Immunology·François JordierJulie Di Cristofaro
Jul 13, 2017·Immunogenetics·Gry PerssonThomas Vauvert F Hviid
Feb 9, 2021·Frontiers in Immunology·Claudio AnnaloroFrancesco Onida
Aug 20, 2019·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Chrysanthi TsamadouJoannis Mytilineos
May 12, 2019·Molecular Immunology·Julien PaganiniJulie Di Cristofaro
Apr 17, 2021·Frontiers in Immunology·Gry PerssonJulie Di Cristofaro
Sep 27, 2020·Cellular Immunology·Jason R Lees
Dec 7, 2021·PLoS Neglected Tropical Diseases·Hannes VietzenElisabeth Puchhammer-Stöckl

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