Peptide Microarray-Based Metal Enhanced Fluorescence Assay for Multiple Profiling of Matrix Metalloproteinases Activities

Analytical Chemistry
Zhen LeiZhenxin Wang

Abstract

Matrix metalloproteinases (MMPs) are closely associated with cancer cell invasion and metastasis. Herein, a fluorescence resonance energy transfer (FRET)-peptide microarray-based metal enhanced fluorescence (MEF) assay is proposed for multiple and sensitive profiling of MMPs activities on a novel Au/Ag@SiO2 substrate. The Au/Ag@SiO2 substrate is prepared by electroless deposition of silver on gold nanoparticle (GNP) seeds, followed by SiO2 shell coating and surface functionalization. The specific FRET peptides are spotted on the Au/Ag@SiO2 substrate to sensitively detect MMPs (MMP-2, -3, -7, -9, -14) via fluorescence recovery by the MMP cleavage of quenched peptide motifs and further enhanced by MEF. Under the optimal conditions, the limits of detection are 12.2 fg mL-1 for MMP-2, 60 pg mL-1 for MMP-3, 0.22 pg mL-1 for MMP-7, 102 fg mL-1 for MMP-9, and 0.68 ng mL-1 for MMP-14, respectively. The practicability of the FRET-peptide microarray-based MEF assay is demonstrated by profiling of multiplexed MMPs activities in various cell lines and clinical thyroid tissue samples of papillary thyroid carcinoma (PTC) patients and thyroid nodules (TN) patients, and satisfactory results are obtained.

References

Jul 27, 1999·The Journal of Biological Chemistry·H Nagase, J F Woessner
May 7, 2002·Nature Reviews. Cancer·Mikala Egeblad, Zena Werb
Dec 12, 2002·Journal of the American Chemical Society·Cleo M SalisburyJonathan A Ellman
Apr 4, 2003·Analytical Biochemistry·Joanna MalickaJoseph R Lakowicz
Nov 27, 2004·Chemistry : a European Journal·Benjamin WileyYounan Xia
May 9, 2006·Cancer Metastasis Reviews·Elena I Deryugina, James P Quigley
Nov 17, 2006·The Journal of Physical Chemistry. B·Fang XieEwa M Goldys
Dec 16, 2006·Nucleic Acids Research·Chandran R Sabanayagam, Joseph R Lakowicz
Oct 6, 2007·International Journal of Cancer. Journal International Du Cancer·Dafeng YangKebin Liu
Jan 4, 2008·International Journal of Cancer. Journal International Du Cancer·Zheng-Sheng WuXiao-Chun Xu
Jul 16, 2008·Molecular Aspects of Medicine·Gillian Murphy, Hideaki Nagase
Aug 8, 2008·Biosensors & Bioelectronics·B Ramana MurthyW T Liu
Nov 10, 2009·Journal of Cancer Research and Clinical Oncology·Xingjun YaoGuojin Liu
Apr 1, 2010·Analytical Chemistry·Olivier R BolducJean-François Masson
Sep 18, 2010·Current Medicinal Chemistry·O ZitkaR Kizek
Dec 25, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Miles A MillerDouglas A Lauffenburger
Mar 15, 2011·Journal of Hazardous Materials·E SumeshT Pradeep
May 14, 2011·Clinical Biochemistry·Suprava PatelAlpana Saxena
Sep 20, 2011·Journal of Agricultural and Food Chemistry·Meng-Chiu LinFeng-Yao Tang
Oct 22, 2011·Molecular Pharmaceutics·Lei ZhuXiaoyuan Chen
Dec 14, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Chunyuan SongYiping Zhao
May 1, 2012·Biosensors & Bioelectronics·Deok-Hoon KongKwon-Soo Ha
Dec 30, 2014·Analytical Chemistry·Tushar D RaneTza-Huei Wang
Jan 20, 2015·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Biebele AbelKadir Aslan
May 23, 2015·Theranostics·Yaping WangYongzhuo Huang
Aug 26, 2015·The Onderstepoort Journal of Veterinary Research·Osama M IshagYahia H Ali
Oct 21, 2015·Journal of the American Chemical Society·Chao-Yu LiZhong-Qun Tian
Aug 18, 2016·ACS Applied Materials & Interfaces·Bei-Bei KouRuo Yuan

❮ Previous
Next ❯

Related Concepts

Related Papers

Vojnosanitetski pregled. Military-medical and pharmaceutical review
Elena KostovaVesna Janevska
International Journal of Urology : Official Journal of the Japanese Urological Association
Masayuki TakahashiSusumu Kagawa
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
K Imai
© 2022 Meta ULC. All rights reserved