PMID: 15344223Sep 3, 2004Paper

Peptide mimotopes of Neisseria meningitidis group B capsular polysaccharide

Yonsei Medical Journal
In Ho ParkJeon-Soo Shin

Abstract

The antigenic similarity between Neisseria meningitidis group B (NMGB) capsular polysaccharide (PS) and human polysialic acid (PSA) has hampered the development of a NMGB PS-based vaccine. But the possibility of a safe vaccine based on NMGB PS has been demonstrated by the existence of the NMGB PS-associated nonautoreactive epitope, which is distinct from those present on human PSA. To obtain peptide mimotopes of NMGB PS, we used HmenB3, a protective and nonautoreactive monoclonal antibody, to screen a phage library with 12 amino acids. We obtained 23 phage clones that bound to HmenB3 but not in the presence of E. coli K1 PS [which is alpha(2-8)-linked PSA like NMGB PS]. The clones contained 3 mimotopes and differed from previously described NMGB PS mimotopes. Immunization with a synthetic peptide of one mimotope elicited anti-NMGB antibodies in BALB/c mice. These mimotopes may be useful in the development of group B meningococcal vaccines.

Citations

Jan 25, 2011·Microbial Biotechnology·Ulrich Vogel, Heike Claus
Feb 8, 2006·Trends in Microbiology·Lisa M MullenBrian Henderson
Jul 30, 2014·Frontiers in Immunology·Thomas Kieber-EmmonsRamachandran Murali
Sep 30, 2016·Journal of Biomedical Science·Leili Aghebati-MalekiJafar Majidi
Apr 21, 2007·Journal of Medical Microbiology·Ayelet BarenholzHervé Bercovier

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