Peptide mimotopes selected from a random peptide library for diagnosis of Epstein-Barr virus infection

Journal of Clinical Microbiology
J L CaseyM Foley

Abstract

Epstein-Barr virus (EBV) is a ubiquitous, worldwide infectious agent that causes infectious mononucleosis, affecting >90% of the world's population. Currently, enzyme-linked immunosorbent assay, mostly with purified preparations of EBV cell extracts to capture immunoglobulin M (IgM) antibodies in patients' serum, is used for primary diagnosis. Our objective was to determine whether a small set of peptides could contain sufficient immunogenic information to replace solid-phase antigens in EBV diagnostics. Using monoclonal antibodies, we selected four peptides that mimic different epitopes of EBV from a phage-displayed random peptide library. To assess their diagnostic value, we screened a panel of 62 individual EBV IgM sera for their reactivities with the peptides alone. For all peptides, there was a clear distinction between the EBV-positive and the EBV-negative samples, resulting in 100% specificity. The sensitivities were 88%, 85%, 71%, and 54% for peptides F1, A3, gp125, and A2, respectively. Any combination of peptides increased the sensitivity, indicating that individual peptides react with different subsets of antibodies. Furthermore, when the F1 and the gp125 peptides were coupled to bovine serum albumin and screened aga...Continue Reading

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Citations

Jun 15, 2011·Protein Engineering, Design & Selection : PEDS·J L CaseyM Foley
Oct 22, 2008·Current Opinion in Chemical Biology·Jutta Eichler
Jun 18, 2009·ChemMedChem·Christoph KesselChristoph Königs
Sep 11, 2018·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Jan PavlicekEva Klaskova
Mar 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kuei-Chen ChiangShuji Sato
Aug 28, 2020·The Journal of Biological Chemistry·Kwabena F M OpuniMichael O Glocker
Dec 17, 2020·Pharmaceutics·Luíse L ChavesSalette Reis
Jun 2, 2018·Talanta·Juliana F de SantanaJuliana de Moura
May 29, 2019·Journal of Proteome Research·Pablo San Segundo-AcostaRodrigo Barderas

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