Peptide modified paper based impedimetric immunoassay with nanocomposite electrodes as a point-of-care testing of Alpha-fetoprotein in human serum

Biosensors & Bioelectronics
Maryam MoazeniAhmad Kermanpur

Abstract

Treatment for cancer depends on the type of cancer, and the stage or its development, and thus the need for point-of-care technology that can allow rapid and precise detection of biomarkers is increasing. Here, we present a simple on chip electrical detection of Alpha-fetoprotein (AFP). We rely on using a novel peptide modified plastic-paper microfluidic chips to perform efficient and specific impedimetric detection of AFP in human serum. The chips are prepared from a lower sheet of plastic and upper layer of cellulose chromatography paper modified with silver-20 wt% graphene printed electrodes. Diphenylalanine (FF) was proposed to involve in detection zone of the fabricated microchips in order to improve the sensing performance and the stability of immobilized antibodies according to amine-aldehyde reaction. The target protein is captured on the surface of microchips using specific monoclonal antibodies and the electrical response of the chip is monitored in the presence and absence of different concentrations of AFP. The influence of several parameters including the material types for screen printing of electrodes, FF concentrations, solvent and pH of FF solution on electrical response and cellulose fibers morphology was expl...Continue Reading

Citations

Feb 15, 2020·Sensors·Manuel Gutiérrez-CapitánCésar Fernández-Sánchez
Oct 16, 2019·Electrophoresis·Worapot Suntornsuk, Leena Suntornsuk
Jul 15, 2020·Biosensors·Paloma Yáñez-SedeñoJosé Manuel Pingarrón
Apr 22, 2021·Lab on a Chip·Wen-Chin LeeLung-Ming Fu
Nov 19, 2021·Biomedical Microdevices·Vinay KishnaniAnkur Gupta

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