Peptide nucleic acid clamp PCR: a novel K-ras mutation detection assay for colorectal cancer micrometastases in lymph nodes

International Journal of Cancer. Journal International Du Cancer
Bret TabackDave S B Hoon

Abstract

Inaccurate staging of colorectal cancer (CRC) has been attributed to the failure to detect lymph node metastases by conventional pathology. We have previously reported the use of lymphatic mapping to accurately identify those lymph nodes most likely to harbor micrometastatic disease and permit focused pathologic examination. Mutation of K-ras allele at codons 12 or 13 occurs frequently in early stages of CRC development. The purpose of our study was to assess sentinel lymph nodes (SLN) for occult CRC micrometastases using a unique peptide nucleic acid (PNA) clamp PCR assay specific for K-ras mutations. Seventy-two paraffin-embedded primary CRC and paired SLN were evaluated by PNA clamp PCR for K-ras mutations. Thirty primary tumors (42%) were positive for K-ras mutations, and in 5 of these cases the SLN were positive for metastases by Hematoxylin and Eosin staining. PNA clamp PCR identified occult metastases in an additional 6 patients, upstaging 24% of K-ras positive primary CRCs (p = 0.014). No K-ras mutations were detected among the 20 noncancer lymph nodes assessed. This study demonstrates the utility, specificity and sensitivity of PNA clamp PCR assay in identifying occult micrometastases in the SLN of CRC patients by sing...Continue Reading

References

Apr 1, 1992·Archives of Surgery·D L MortonA J Cochran
Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L LosiJ Costa
Apr 1, 1991·Cancer·A M CohenE R Sigurdson
Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Nov 1, 1989·The British Journal of Surgery·K W Scott, R H Grace
Sep 1, 1988·The New England Journal of Medicine·B VogelsteinJ L Bos
Mar 1, 1988·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C ErlichmanT Elhakim
Jan 1, 1986·The British Journal of Surgery·S J CawthornC G Marks
Jul 1, 1986·International Journal of Colorectal Disease·J R JassJ M Northover
Oct 1, 1982·Pathology, Research and Practice·P Schmitz-MoormannR Söhl
Sep 1, 1994·Annals of Surgery·A E GiulianoD L Morton
Jul 7, 1993·Journal of the National Cancer Institute·D ShibataM Perucho
Dec 1, 1995·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·B R DixB J Iacopetta
Aug 15, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P J BostickD S Hoon
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J H WongT S Namiki
Mar 4, 2000·The Journal of Pathology·J W Arends
Jun 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Ray, B Nordén
Jun 2, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S TórtolaM A Reymond
Sep 5, 2001·British Journal of Cancer·H J AndreyevN Urosevic

❮ Previous
Next ❯

Citations

May 17, 2011·International Journal of Colorectal Disease·Satu OltedalOddmund Nordgård
Apr 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masaru ShinozakiDave S B Hoon
Jun 12, 2009·BMC Cancer·Ya-Sian ChangJan-Gowth Chang
Mar 3, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Raffaele Di FranciaPamela Pinzani
Dec 26, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Kuo-Chien TsaoShin-Ru Shih
Apr 5, 2011·Journal of the American College of Surgeons·Zhenbin ChenJulio Garcia-Aguilar
Oct 4, 2008·The Journal of Molecular Diagnostics : JMD·Kenji TatsumiYoshihide Hayashizaki
Jun 17, 2008·The Journal of Molecular Diagnostics : JMD·Bjørnar GiljeOddmund Nordgård
Mar 10, 2011·International Journal of Cancer. Journal International Du Cancer·Sascha TierlingJörn Walter
Dec 8, 2011·Molecular Ecology Resources·R O'RorkeA Jeffs
Jan 27, 2015·Surgery·Nakul P ValsangkarSarah P Thayer
Nov 16, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberta D'AgataGiuseppe Spoto
Feb 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Munira F Fouz, Daniel H Appella

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.