Peptide nucleic acid films and capsules: assembly and enzymatic degradation

Macromolecular Bioscience
Alisa L BeckerFrank Caruso

Abstract

Sequence-directed hybridization of nucleic acids provides a high level of control for the bottom-up assembly of nanostructured materials. Altering the DNA sequence affords control and versatility over the film structure, but is limited by the chemical and physical properties of DNA. Here, we use DNA analogues, peptide nucleic acids (PNAs), to introduce new properties to multilayered thin films and retain the advantages of sequence-directed assembly. Thin films, formed by the layer-by-layer (LbL) assembly of PNA strands, were assembled from short PNA sequences on planar and colloidal substrates. In the case of PNA-coated particles, hollow capsules were obtained following removal of the sacrificial particle template. The PNA films were stable to both nuclease and protease degradation, and the nuclease degradation rate could be tuned by varying the amount of DNA incorporated into the films. These thin films may find use in biomedical applications.

References

Dec 22, 1986·Nucleic Acids Research·R I MaN C Seeman
Sep 15, 1994·Biochemical Pharmacology·V V DemidovP E Nielsen
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·O Almarsson, T C Bruice
May 1, 1996·Nature Structural Biology·M Eriksson, P E Nielsen
Aug 21, 2003·Journal of the American Chemical Society·Rong CaoBruce A Armitage
Mar 17, 2006·Nature·Paul W K Rothemund
Mar 22, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Angus P R JohnstonFrank Caruso
Apr 9, 2008·Small·Angus P R Johnston, Frank Caruso
Mar 13, 2008·Science and Technology of Advanced Materials·Katsuhiko ArigaSomobrata Acharya
Sep 4, 1998·Angewandte Chemie·Edwin DonathHelmuth Möhwald

❮ Previous
Next ❯

Citations

Sep 20, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Benjamin M Wohl, Johan F J Engbersen
Jun 28, 2011·Advanced Drug Delivery Reviews·Melgardt M de VilliersYuri M Lvov
Nov 1, 2012·Chemical Communications : Chem Comm·Peter N Brown, Hang Yin
May 12, 2015·Artificial Cells, Nanomedicine, and Biotechnology·Sripriya Jaganathan
Jan 5, 2021·ACS Applied Materials & Interfaces·Margarita Vázquez-González, Itamar Willner
Mar 19, 2011·Analytical Chemistry·Z AliW J Parak
Oct 20, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Khalil AbdelkebirGuy Ladam
Aug 21, 2014·Chemical Reviews·João Borges, João F Mano
Aug 18, 2010·Small·Alisa L BeckerFrank Caruso
Oct 1, 2010·Chemical Society Reviews·Georgina K SuchFrank Caruso

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved