Peptide nucleic acid-mediated competitive PCR clamping for detection of rifampin-resistant Mycobacterium tuberculosis

Antimicrobial Agents and Chemotherapy
Tomotada Iwamoto, Toshiaki Sonobe

Abstract

Peptide nucleic acid-mediated competitive PCR clamping, which can selectively amplify mutant alleles, was developed to detect mutations in four codons (513, 516, 526, and 531) of the rpoB gene in Mycobacterium tuberculosis strains. This simple method successfully identified the mutations in all 40 of the M. tuberculosis strains tested.

References

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Apr 1, 1994·Antimicrobial Agents and Chemotherapy·L P MillerT M Shinnick
Oct 1, 1995·Clinical Microbiology Reviews·J M Musser
Mar 21, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D L WilliamsD H Persing
Jan 25, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·S Ramaswamy, J M Musser

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Citations

Oct 5, 2007·Expert Review of Anti-infective Therapy·Alberto MatteelliMario Raviglion
May 15, 2008·The Annals of Pharmacotherapy·Shawn D Anderson, John G Gums

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