PMID: 9443962Mar 14, 1998Paper

Peptide nucleic acid (PNA)/DNA hybrid duplexes: intercalation by an internally linked anthraquinone

Nucleic Acids Research
B ArmitageG B Schuster

Abstract

Peptide nucleic acids (PNA) mimic DNA and RNA by forming complementary duplex structures following Watson-Crick base pairing. A set of reporter compounds that bind to DNA by intercalation are known, but these compounds do not intercalate in PNA/DNA hybrid duplexes. Analysis of the hybrid PNA duplexes requires development of reporter compounds that probe their chemical and physical properties. We prepared a series of anthraquinone (AQ) derivatives that are linked to internal positions of a PNA oligomer. These are the first non-nucleobase functional groups that have been incorporated into a PNA. The resulting PNA(AQ) conjugates form stable hybrids with complementary DNA oligomers. We find that when the AQ groups are covalently bound to PNA that they stabilize the hybrid duplex and are, at least partially, intercalated.

References

Nov 27, 1992·Science·J C HanveyK G Au
Dec 11, 1994·Nucleic Acids Research·P WittungB Nordèn
May 1, 1996·Nature Structural Biology·M Eriksson, P E Nielsen
Aug 1, 1997·Antisense & Nucleic Acid Drug Development·L Good, P E Nielsen
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·B ArmitageG B Schuster

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Citations

Nov 26, 2003·Biochemical and Biophysical Research Communications·Anders Fuglsang
Jun 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Ray, B Nordén
Apr 9, 2008·Inorganic Chemistry·Konstantinos GkionisJ Grant Hill

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