Peptide-nucleotide microdroplets as a step towards a membrane-free protocell model

Nature Chemistry
Shogo KogaStephen Mann

Abstract

Although phospholipid bilayers are ubiquitous in modern cells, their impermeability, lack of dynamic properties, and synthetic complexity are difficult to reconcile with plausible pathways of proto-metabolism, growth and division. Here, we present an alternative membrane-free model, which demonstrates that low-molecular-weight mononucleotides and simple cationic peptides spontaneously accumulate in water into microdroplets that are stable to changes in temperature and salt concentration, undergo pH-induced cycles of growth and decay, and promote α-helical peptide secondary structure. Moreover, the microdroplets selectively sequester porphyrins, inorganic nanoparticles and enzymes to generate supramolecular stacked arrays of light-harvesting molecules, nanoparticle-mediated oxidase activity, and enhanced rates of glucose phosphorylation, respectively. Taken together, our results suggest that peptide-nucleotide microdroplets can be considered as a new type of protocell model that could be used to develop novel bioreactors, primitive artificial cells and plausible pathways to prebiotic organization before the emergence of lipid-based compartmentalization on the early Earth.

References

Dec 1, 1977·Origins of Life·M P Kolesnikov, I A Egorov
Sep 5, 1978·Biochemistry·W R Hargreaves, D W Deamer
Jan 1, 1991·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·I BaezaJ Oró
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G Wächtershäuser
Jan 1, 1989·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·D W Deamer, R M Pashley
Feb 6, 1995·Biochemical and Biophysical Research Communications·T OberholzerC K Biebricher
Mar 8, 1996·The Journal of Biological Chemistry·J PeltaJ L Sikorav
Oct 1, 1995·Chemistry & Biology·T OberholzerP L Luisi
May 5, 1999·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·T M McCollomB R Simoneit
Feb 24, 2001·Nature·J W SzostakP L Luisi
Sep 29, 2001·Biomolecular Engineering·P Walde, S Ichikawa
Oct 30, 2001·Molecular and Cellular Biochemistry·A Ehsani-ZonouzM Nemat-Gorgani
Nov 13, 2003·Chembiochem : a European Journal of Chemical Biology·Shin-ichiro M NomuraKenichi Yoshikawa
Dec 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Vincent Noireaux, Albert Libchaber
Jun 6, 2008·Nature·Sheref S MansyJack W Szostak
Jan 9, 2009·Angewandte Chemie·Atul AsatiJ Manuel Perez
Mar 11, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Peter Walde
May 4, 2010·Angewandte Chemie·Uwe J MeierhenrichJason P Dworkin
May 21, 2010·Cold Spring Harbor Perspectives in Biology·Jason P SchrumJack W Szostak

❮ Previous
Next ❯

Citations

Mar 13, 2012·Accounts of Chemical Research·Stephen Mann
Oct 24, 2012·Nature Chemistry·Christopher A StrulsonPhilip C Bevilacqua
Dec 26, 2013·The Journal of Cell Biology·Clifford P Brangwynne
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina SokolovaWilhelm T S Huck
Mar 13, 2014·Annual Review of Biochemistry·J Craig Blain, Jack W Szostak
May 2, 2014·Nature Methods·James Attwater, Philipp Holliger
Mar 1, 2014·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Tony Z JiaJack W Szostak
Jun 22, 2014·Current Opinion in Chemical Biology·Mei LiStephen Mann
Sep 12, 2014·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Richard Egel
Jan 15, 2015·Nature Communications·Sarah L PerryMatthew Tirrell
Feb 5, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Erica A FrankelChristine D Keating
Jan 23, 2016·Nature Chemistry·William M Aumiller, Christine D Keating
Mar 15, 2016·Current Opinion in Biotechnology·Eliza MorrisCheemeng Tan
Nov 21, 2015·Biophysical Journal·Bradley W DavisChristine D Keating
Apr 14, 2015·Life·Pierre-Alain Monnard, Peter Walde
May 11, 2016·Advances in Colloid and Interface Science·Charles E Sing
Jun 9, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Nicolas MartinStephen Mann
Jul 16, 2016·Advances in Colloid and Interface Science·Amanda B MarcielLorraine Leon
Sep 17, 2016·Advances in Colloid and Interface Science·Yalin LiuSarah L Perry
Sep 7, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·William M AumillerChristine D Keating
Nov 5, 2016·Protein Science : a Publication of the Protein Society·Ciara KynePeter B Crowley
Apr 1, 2017·Chemical Communications : Chem Comm·Marta Sobrinos-SanguinoGermán Rivas
Sep 24, 2016·Angewandte Chemie·David GarenneJean-Paul Douliez
Nov 13, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·James FothergillStephen Mann
Apr 22, 2015·Chemical Communications : Chem Comm·Kangle LvStephen Mann
Jun 19, 2015·Chemical Communications : Chem Comm·T-Y Dora TangStephen Mann
May 7, 2016·Physical Chemistry Chemical Physics : PCCP·Lorenz KeilDieter Braun
Jun 29, 2017·ACS Nano·Tatiana TrantidouOscar Ces
Jun 29, 2017·Angewandte Chemie·Nan-Nan Deng, Wilhelm T S Huck
Jun 21, 2017·Soft Matter·Benxin JingYingxi Zhu
Feb 2, 2016·Journal of Colloid and Interface Science·David GarenneJean-Paul Douliez
Mar 27, 2018·Chemical Communications : Chem Comm·B V V S Pavan KumarStephen Mann
Dec 17, 2014·Chemical Communications : Chem Comm·Lin NiuDehai Liang
Aug 16, 2017·Physical Chemistry Chemical Physics : PCCP·Wei PanDehai Liang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.