Peptide-phospholipid complex formation at liquid-liquid interfaces.

Analytical Chemistry
Manuel A MéndezHubert H Girault

Abstract

Two peptides known to interact with receptors embedded in cell membranes, angiotensin III (Ang III) and Leu-enkephalin (LeuEnk), were studied electrochemically at the interface formed between two immiscible electrolyte solutions modified by an adsorbed monolayer of dipalmitoylphosphatidylcholine (DPPC). The results indicate that cationic angiotensin III transfer can be facilitated by the interfacial formation of a complex with DPPC. The complexation constant was determined by voltammetry and found to be equal to 5.2 x 10(4) M(-1). For neutral Leu-enkephalin, a current only observable in the presence of the lipidic monolayer results from the formation of a complex between the lithium cation, LeuEnk or LeuEnk dimer and the phospholipid. For both peptides, the peptide-lipid complexes were identified by biphasic electrospray ionization mass spectrometry using a setup consisting of a dual-channel microchip, which puts in contact two immiscible phases at the Taylor cone and makes possible the study of interfacial complexes. The stability of the 1:1 complexes between lysine, diphenylalanine, Ang III, and LeuEnk and DPPC were evaluated by varying the temperature of the heated capillary of the mass spectrometer. Finally, from the comple...Continue Reading

Citations

Feb 7, 2013·Journal of the American Society for Mass Spectrometry·Yu LuHubert H Girault
Oct 26, 2013·Journal of Agricultural and Food Chemistry·Paolo LucciGian Luigi Gianfranceschi
Oct 4, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Manuel A MéndezHubert H Girault
Aug 6, 2014·Biochimica Et Biophysica Acta·Seza ErgunFeride Severcan
Sep 16, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Stefano PerniValeria Marsili
Jun 10, 2010·European Journal of Mass Spectrometry·Hubert H GiraultMélanie Abonnenc
Dec 12, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Pekka PeljoKyösti Kontturi
Oct 20, 2009·The Analyst·Michel Prudent, Hubert H Girault
Nov 28, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Hélder A SantosCarlos M Pereira
Nov 13, 2010·Metallomics : Integrated Biometal Science·Michel PrudentHubert H Girault
Jul 26, 2012·Chemistry, an Asian Journal·Eva Alvarez de EulateDamien W M Arrigan

Related Concepts

Phenylalanylphenylalanine monohydrochloride, (L,L)-isomer
Diphenylalanine
Angiotensin III
Electrochemistry
Enkephalin, Leucine
Lithium
Lysine Hydrochloride
Peptide Fragments
Endorphenyl
1,2-Dipalmitoylphosphatidylcholine

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