Peptide profiling by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of the Lasiodora parahybana tarantula venom gland

Toxicon : Official Journal of the International Society on Toxinology
Catherine GuetteMarie-Louise Célérier

Abstract

In order to establish a venom fingerprint and a peptide profile of the Lasiodora parahybana tarantula venom gland, we used conventional methods such as reversed phase liquid chromatography coupled to an electrospray-ionisation hybrid quadrupole time of flight mass spectrometer (LC/ESI-QqTOFMS), matrix-assisted laser desorption/ionization time-of-flight-MS (MALDI-TOFMS) and direct study of L. parahybana venom by nanospray-ionization QqTOFMS (nanoESI-QqTOFMS) and a new technology for the direct analysis of fresh tissues using MALDI-TOFMS. The analysis of the crude venom allowed the characterization of specific juvenile and adult biomarkers. In situ MALDI analysis of L. parahybana venom gland sections revealed different peptide expression levels all along the gland and non-processed peptide precursors, demonstrating the power of the method for the dynamic investigation of peptide evolution in the venom gland of spiders.

References

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Citations

Jan 10, 2009·Cell and Tissue Research·Thomaz A A Rocha-e-SilvaStephen Hyslop
Apr 8, 2010·Cellular and Molecular Life Sciences : CMLS·Elia Diego-GarcíaJan Tytgat
Jun 1, 2007·Expert Opinion on Drug Discovery·Pierre Escoubas, Frank Bosmans
Jul 25, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Armando A RodríguezAbilio Laguna
Apr 21, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Liping JiangSongping Liang
Nov 27, 2008·Toxicon : Official Journal of the International Society on Toxinology·Thomaz A A Rocha-E-SilvaStephen Hyslop
Sep 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·André Junqueira ZaharenkoJosé Carlos de Freitas
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Nov 28, 2012·Toxicon : Official Journal of the International Society on Toxinology·Caroline Barbosa F MourãoElisabeth F Schwartz
Jun 25, 2013·Toxicon : Official Journal of the International Society on Toxinology·C C HortaE Kalapothakis
Oct 30, 2016·Toxicon : Official Journal of the International Society on Toxinology·Felipe Roberto Borba FerreiraPatrícia Maria Guedes Paiva

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