Peptide separation by Hydrophilic-Interaction Chromatography: a review

Journal of Biochemical and Biophysical Methods
Tatsunari Yoshida

Abstract

Recent developments in the separation of peptides by high-performance liquid chromatography (HPLC) using polar sorbents with less polar eluents are summarized in this review. This separation mode is now commonly referred to as Hydrophilic-Interaction Chromatography (HILIC). The retention mechanism and chromatographic behavior of polar solutes under HILIC conditions are studied on TSKgel Amide-80 columns, which consist of carbamoyl groups bonded to a silica gel matrix, using a mixture of acetonitrile (MeCN)-water containing 0.1% trifluoroacetic acid (TFA). Some applications are given in peptide field using Hydrophilic-Interaction Chromatography.

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Citations

Feb 1, 2011·Analytical and Bioanalytical Chemistry·Ana ReisM Rosário M Domingues
Sep 1, 2011·Analytical and Bioanalytical Chemistry·Bogusław Buszewski, Sylwia Noga
Apr 15, 2008·Molecular & Cellular Proteomics : MCP·Claudio P AlbuquerqueHuilin Zhou
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Oct 16, 2015·Analytical and Bioanalytical Chemistry·Sarah RogstadMichael T Boyne
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Dec 3, 2016·Analytical and Bioanalytical Chemistry·Kathirvel AlagesanDaniel Kolarich
Apr 4, 2017·Electrophoresis·Lucas VeillonYehia Mechref
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