Peptide-Stabilized Emulsions and Gels from an Arginine-Rich Surfactant-like Peptide with Antimicrobial Activity

ACS Applied Materials & Interfaces
Valeria CastellettoJanne Ruokolainen

Abstract

The preparation of hydrogels and stable emulsions is important in the formulation of many functional nanostructured soft materials. We investigate the multifunctional self-assembly and bioactivity properties of a novel surfactant-like peptide (SLP) that shows antimicrobial activity, is able to form hydrogels without pH adjustment, and is able to stabilize oil-in-water emulsions. Furthermore, we demonstrate on-demand de-emulsification in response to the protease enzyme elastase. We show that SLP (Ala)9-Arg (A9R) forms β-sheet fibers above a critical aggregation concentration and that water-in-oil emulsions are stabilized by a coating of β-sheet fibers around the emulsion droplets. Furthermore, we demonstrate enzyme-responsive de-emulsification, which has potential in the development of responsive release systems. The peptide shows selective antimicrobial activity against Gram-negative pathogens including Pseudomonas aeruginosa, which causes serious infections. Our results highlight the utility of SLPs in the stabilization of oil/water emulsions and the potential for these to be used to formulate antimicrobial peptide emulsions which are additionally responsive to protease. The peptide A9R has pronounced antibacterial activity ag...Continue Reading

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Citations

Feb 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lucas R MelloEmerson R Silva
Oct 10, 2020·Chemical Communications : Chem Comm·Valeria CastellettoIan W Hamley
May 1, 2021·International Journal of Molecular Sciences·Fabian RicardoOscar Alvarez
Mar 21, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Abhijit SahaMeital Reches
Aug 14, 2019·ACS Applied Materials & Interfaces·Valeria CastellettoJanne Ruokolainen
Mar 7, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Juliane N B D PelinWendel A Alves
Mar 7, 2020·ACS Applied Materials & Interfaces·Juliane N B D PelinIan W Hamley
Jun 4, 2019·ACS Applied Bio Materials·Charlotte J C Edwards-GayleJanne Ruokolainen
Apr 17, 2020·ACS Applied Bio Materials·Charlotte J C Edwards-GayleIan W Hamley
Dec 16, 2020·ACS Biomaterials Science & Engineering·Neelam Chauhan, Yashveer Singh
Aug 24, 2021·Colloids and Surfaces. B, Biointerfaces·Chunqian ZhaoXinke Zhang
Mar 21, 2020·ACS Applied Materials & Interfaces·Ruilian QiYilin Wang
Sep 15, 2021·Chemical Reviews·Fahmeed SheehanRein V Ulijn

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Methods Mentioned

BETA
X-ray
circular dichroism
transmission
fluorescence assay
Infrared Spectroscopy
differential
confocal microscopy
fluorescence
rheology
optical microscopy

Software Mentioned

SASfit
ISPyB
ScÅtter

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