Peptide YY attenuates STAT1 and STAT3 activation induced by TNF-alpha in acinar cell line AR42J

Journal of the American College of Surgeons
Kristine RobinsonDavid McFadden

Abstract

STAT1 and STAT3, members of the cytoplasmic family of signal transducers and activators of transcription factors (STAT), have been associated with numerous inflammatory pathologies, including inflammatory bowel disease, hepatitis, and acute lung injury. But little is known about their role in the pancreas. Peptide YY (PYY), an inhibitory gastrointestinal hormone, ameliorates pancreatitis in vivo and in vitro. In addition, we have shown that PYY attenuates transcription factors, such as nuclear transcription factor (NF)-kappaB and Smad3/4, which mediate inflammation. We hypothesized that tumor necrosis factor (TNF)-alpha would induce STAT1 and STAT3, and PYY would attenuate their transcription factor binding. Rat pancreatic acinar cells were treated with recombinant TNF-alpha (200 ng/mL); PYY (3-36; 500 pM) was added 30 minutes post-TNF-alpha treatment. Cells were harvested at 2 hours, and nuclear protein and conditioned media were extracted. Levels of amylase secretion and cytokine production were measured using commercially available kits. STAT transcription factor binding was determined by protein/DNA array analysis and densitometry; results were verified again by electrophoretic mobility shift assay (EMSA) and ELISA-based as...Continue Reading

References

Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J M LundbergB Hamberger
Jan 28, 1998·American Journal of Surgery·J Norman
Oct 20, 1999·Cellular and Molecular Life Sciences : CMLS·T Decker
Oct 20, 1999·Cellular and Molecular Life Sciences : CMLS·D E Levy
Oct 20, 1999·Cellular and Molecular Life Sciences : CMLS·D J Hilton
May 19, 2000·Cytokine & Growth Factor Reviews·K Takeda, S Akira
May 8, 2002·The Journal of Clinical Investigation·David E Levy, Chien-kuo Lee
Sep 11, 2002·Cytokine & Growth Factor Reviews·Toshitkatsu Hanada, Akihiko Yoshimura
Sep 19, 2003·The Journal of Surgical Research·Kevork K KazanjianDavid W McFadden
Oct 17, 2003·Current Drug Targets. Inflammation and Allergy·L G Gómez-CambroneroJ Sastre
Jan 20, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mariano SevergniniAmy R Simon
Sep 24, 2004·Current Pharmaceutical Design·E PfitznerC M Litterst
Mar 9, 2005·Digestive Diseases·Vuk SavkovićHans Bödeker
Apr 2, 2005·Current Gastroenterology Reports·Albert B LowenfelsPatrick Maisonneuve
Apr 26, 2005·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Madhav BhatiaLakshmi Chevali

❮ Previous
Next ❯

Citations

Oct 29, 2013·Journal of Ethnopharmacology·Li WuBao-chang Cai
Feb 6, 2010·World Journal of Gastrointestinal Pharmacology and Therapeutics·Isabel De Dios
May 8, 2015·Biological & Pharmaceutical Bulletin·Lei ZouJieshou Li
Sep 27, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Dongmei ZhangZhongwei Huang
Dec 7, 2007·The International Journal of Biochemistry & Cell Biology·Shunichi ShimizuYuji Kiuchi
Sep 7, 2018·Experimental and Therapeutic Medicine·Minli LiFangyu Wang
Oct 16, 2012·The American Journal of Pathology·Minoru ShigekawaTetsuo Takehara
May 1, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Zehua ZhouXinhong Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here