Peptide YY attenuates transcription factor activity in tumor necrosis factor-alpha-induced pancreatitis

Journal of the American College of Surgeons
Linda Vona-DavisDavid McFadden

Abstract

Acute pancreatitis (AP) is a disease characterized by inflammation. Nuclear factor (NF)-kappaB, Smad proteins, and the steroid hormone family peroxisome proliferator-activated receptors (PPARs) are involved in regulation of gene transcription during the disease process. Peptide YY (PYY), a gastrointestinal hormone, inhibits NF-kappaB translocation to acinar nuclei in tumor necrosis factor (TNF)-alpha-induced AP. We investigated TNF-alpha induction of Smad proteins, PPARalpha/gamma, and NF-kappaB by TNF-alpha, and hypothesized that PYY would attenuate this effect. Rat acinar cells were treated with recombinant TNF-alpha (200 ng/mL). PYY (3 to 36) was added at 500 pM at 30 minutes after TNF-alpha treatment until cell harvest at 2 hours. Western blot analysis and intracellular staining of the p65 subunit of NF-kappaB were performed. NF-kappaB, Smad3/4, and PPARalpha/gamma binding activities were determined by protein/DNA array analysis and verified by electrophoretic-mobility shift assay and densitometry. Cellular localization of NF-kappaB p65 showed nuclear staining within 2 hours, with controls stained in the cytoplasm. With PYY, p65 stained in the cytoplasm. Nuclear p65 was increased significantly (p < 0.05) by TNF-alpha at 2 h...Continue Reading

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Citations

May 17, 2005·The Journal of Surgical Research·Linda C Vona-DavisDavid W McFadden
Jul 25, 2013·Rheumatology·Chih-Yen Chen, Chang-Youh Tsai
Feb 28, 2006·Journal of the American College of Surgeons·Edgar A Guzman, Marek Rudnicki
Jul 7, 2017·Critical Reviews in Food Science and Nutrition·Lei ChenJianbo Xiao

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