Peptides are building blocks of heat-induced fibrillar protein aggregates of beta-lactoglobulin formed at pH 2

Biomacromolecules
C AkkermansErik van der Linden

Abstract

The proteinaceous material present in beta-lactoglobulin fibrils formed after heating (20 h at 85 degrees C) at pH 2 was identified during this study. Fibrils were separated from the nonaggregated material, and the fibrils were dissociated using 8 M guanidine chloride and 0.1 M 1,4-dithiothreitol (pH 8). Characterization of the different fractions was performed using thioflavin T fluorescence, high-performance size-exclusion chromatography, reversed-phase HPLC, and mass spectrometry (MALDI-TOF). Beta-lactoglobulin was found to be hydrolyzed into peptides with molecular masses between 2000 and 8000 Da, and the fibrils were composed of a part of these peptides and not intact beta-lactoglobulin. The majority of the peptides (both aggregated and nonaggregated) were a result from cleavage of the peptide bonds before or after aspartic acid residues. Explanations for the presence of certain peptide fragments in the fibrils are the hydrophobicity, low charge, charge distribution, and capacity to form beta-sheets.

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P WestermarkC Betsholtz
Jan 1, 1983·Methods in Enzymology·A S Inglis
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Oct 18, 2000·International Journal of Biological Macromolecules·G M KavanaghS B Ross-Murphy
Jan 19, 2002·Nature Structural Biology·Fabrizio ChitiChristopher M Dobson
Jan 31, 2002·Analytical Chemistry·A LiR J Fisher
Sep 19, 2002·Protein Science : a Publication of the Protein Society·Daizo Hamada, Christopher M Dobson
Nov 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Manuela López De La PazLuis Serrano
Jun 12, 2003·Journal of Agricultural and Food Chemistry·Cecile VeermanErik van der Linden
Nov 11, 2003·Biomacromolecules·Luben N ArnaudovCarlo P M van Mierlo
Dec 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Manuela López de la Paz, Luis Serrano
Jul 9, 2004·Journal of Molecular Biology·Erica FrareAngelo Fontana
Jan 5, 2005·Journal of Structural Biology·M R H KrebsA M Donald
Jan 22, 2005·Faraday Discussions·Elizabeth H BromleyAthene M Donald
Jun 19, 2007·Journal of Agricultural and Food Chemistry·Suzanne G BolderErik van der Linden
Oct 2, 2007·Journal of Agricultural and Food Chemistry·Nathalie Creusot, Harry Gruppen

❮ Previous
Next ❯

Citations

Jun 28, 2011·Biomacromolecules·Owen G JonesRaffaele Mezzenga
Sep 8, 2011·Biomacromolecules·Simon M LovedayHarjinder Singh
Jun 12, 2013·Biomacromolecules·Gang Liu, Qixin Zhong
Dec 18, 2013·Biomacromolecules·Anant C DaveHarjinder Singh
Oct 18, 2011·Journal of the American Chemical Society·Corianne C vandenAkkerGijsje H Koenderink
Jul 28, 2011·Journal of Agricultural and Food Chemistry·Libei BatemanHarjinder Singh
Sep 17, 2011·Journal of Agricultural and Food Chemistry·Jin-Mei WangJun-Ru Qi
Dec 2, 2011·Journal of Agricultural and Food Chemistry·Michele Da Silva PintoJoëlle Leonil
Apr 24, 2012·Journal of Agricultural and Food Chemistry·Simon M LovedayHarjinder Singh
Jul 16, 2013·Journal of Agricultural and Food Chemistry·Anant C DaveHarjinder Singh
Dec 25, 2013·Journal of Agricultural and Food Chemistry·Leonardo CornacchiaPaul Venema
Apr 23, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Ardy Kroes-NijboerErik van der Linden
Dec 14, 2012·Faraday Discussions·Ardy Kroes-NijboerErik van der Linden
Feb 26, 2014·Journal of Agricultural and Food Chemistry·Claire Darizu MunialoHarmen H J de Jongh
Mar 14, 2014·Journal of Agricultural and Food Chemistry·Anant C DaveHarjinder Singh
Jun 24, 2014·Journal of Colloid and Interface Science·Skelte G Anema, C G Kees de Kruif
Jan 26, 2016·Nature Nanotechnology·Sreenath Bolisetty, Raffaele Mezzenga
Oct 31, 2015·Soft Matter·Charith A HettiarachchiMartin A K Williams
Feb 18, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Jinfeng PengErik van der Linden
Mar 11, 2016·Soft Matter·Jinfeng PengErik van der Linden
Dec 7, 2013·Journal of the Science of Food and Agriculture·Jian-Zhong ZhouHai-Feng Qian
Apr 7, 2016·Biochimica Et Biophysica Acta·M CarbonaroF Stellato
Jun 13, 2015·Critical Reviews in Food Science and Nutrition·Oscar L RamosAntónio A Vicente
Dec 10, 2008·Journal of Structural Biology·Ardy Kroes-NijboerErik van der Linden
Nov 19, 2011·Food & Function·Ardy Kroes-NijboerErik van der Linden
Jun 7, 2016·Journal of Dairy Science·Hong-Hua XuJun-Yan Tan
Apr 14, 2016·Preventive Nutrition and Food Science·Harjinder Singh
Jun 24, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Alberto Gonzalez-JordanLazhar Benyahia
Jul 28, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Zhili WanLeonard M C Sagis
Aug 4, 2016·The Journal of Physical Chemistry. B·Corianne C VandenAkkerGijsje H Koenderink
Aug 27, 2016·International Journal of Molecular Sciences·Joël ZinkMarkus Schmid
Dec 22, 2016·Critical Reviews in Food Science and Nutrition·Ashkan Madadlou, Alireza Abbaspourrad
Jan 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ayaka KamadaChristofer Lendel
May 1, 2019·Journal of Food Science·Jinfeng PengErik van der Linden
May 11, 2017·Chemical Communications : Chem Comm·Sreenath BolisettyRaffaele Mezzenga
Aug 7, 2020·International Journal of Molecular Sciences·Rebecca RabeTimothy E L Douglas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.