PMID: 9440049Jan 24, 1998Paper

Peptides corresponding to the second epidermal growth factor-like domain of human blood coagulation factor VII: synthesis, folding and biological activity

The Journal of Peptide Research : Official Journal of the American Peptide Society
M HusbynP M Fischer

Abstract

Factor VIIa (FVIIa) is the enzymatically active constituent of the FVIIa/tissue factor (TF) complex, the initiator of the extrinsic pathway of blood coagulation. The zymogen FVII and FVIIa are composed of discrete domains, two of which are homologous to the epidermal growth factor (EGF). This investigation examined the significance of the FVII EGF-2 domain in the processes leading to activation of factor X (FX). Peptides 47 residues in length and corresponding to the amino acid sequence of the EGF-2 domain of human FVII were prepared by solid-phase synthesis methods. Peptide variants with all six Cys residues replaced by L-2-aminobutyryl residues (1), or containing one (2a-c), two (3a,b) or three (4) disulfide bonds, were obtained by application of various S-protecting groups and oxidation methods. Peptide 4, containing the cystine bridge arrangement corresponding to that found in the native protein, was prepared by a two-step regioselective disulfide bond formation method. An evaluation of the anti-coagulant properties of peptides 1-4 revealed that all peptides, with the exception of the two-cystine isomer containing non-native disulfide pairings (3b), were potent inhibitors of TF/FVIIa-mediated activation of FX. The fully con...Continue Reading

References

May 1, 1991·International Journal of Peptide and Protein Research·N V McFerranF C Scott
Sep 1, 1990·International Journal of Peptide and Protein Research·D S KingG B Fields
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P WildgooseW Kisiel
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P J O'HaraM J Murray
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F S HagenK Kurachi
Oct 15, 1993·European Journal of Biochemistry·A Kumar, D S Fair
Apr 1, 1997·The Journal of Peptide Research : Official Journal of the American Peptide Society·M EngebretsenP M Fischer
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

❮ Previous
Next ❯

Citations

Jul 5, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·C K HuP M Fischer
Jul 10, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·Mette Husbyn, Alan Cuthbertson
May 12, 2012·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Hye In WooHee-Jin Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.