Peptides Derived from Mycobacterium leprae ML1601c Discriminate between Leprosy Patients and Healthy Endemic Controls.

Journal of Tropical Medicine
Kidist BoboshaAnnemieke Geluk

Abstract

The stable incidence of new leprosy cases suggests that transmission of infection continues despite worldwide implementation of MDT. Thus, specific tools are needed to diagnose early stage Mycobacterium leprae infection, the likely sources of transmission. M. leprae antigens that induce T-cell responses in M. leprae exposed and/or infected individuals thus are major targets for new diagnostic tools. Previously, we showed that ML1601c was immunogenic in patients and healthy household contacts (HHC). However, some endemic controls (EC) also recognized this protein. To improve the diagnostic potential, IFN-γ responses to ML1601c peptides were assessed using PBMC from Brazilian leprosy patients and EC. Five ML1601c peptides only induced IFN-γ in patients and HHC. Moreover, 24-hour whole-blood assay (WBA), two ML1601c peptides could assess the level of M. leprae exposure in Ethiopian EC. Beside IFN-γ, also IP-10, IL-6, IL-1β, TNF-α, and MCP-1 were increased in EC from areas with high leprosy prevalence in response to these ML1601c peptides. Thus, ML1601c peptides may be useful for differentiating M. leprae exposed or infected individuals and can also be used to indicate the magnitude of M. leprae transmission even in the context of ...Continue Reading

References

Dec 13, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·John S SpencerMaria C V Pessolani
Apr 15, 2006·Clinical Microbiology Reviews·D M ScollardD L Williams
May 27, 2006·Human Immunology·Annemieke Geluk, Tom H M Ottenhoff
Jan 30, 2009·Clinical and Vaccine Immunology : CVI·Annemieke GelukUNKNOWN IDEAL Consortium
Apr 30, 2010·Clinical and Vaccine Immunology : CVI·Annemieke GelukTom H M Ottenhoff
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Elizabeth A MischThomas R Hawn
May 28, 2011·The Lancet Infectious Diseases·Laura C Rodrigues, Diana Nj Lockwood

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Citations

Mar 13, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R M OliveiraM M A Stefani
Mar 30, 2013·Expert Opinion on Medical Diagnostics·Annemieke Geluk
Aug 11, 2016·Diagnostic Microbiology and Infectious Disease·Aline Araújo FreitasMariane Martins Araújo Stefani
Oct 30, 2015·BMC Infectious Diseases·Saraswoti KhadgeAnnemieke Geluk
Jan 11, 2013·Memórias do Instituto Oswaldo Cruz·Kidist BoboshaAnnemieke Geluk
Jan 9, 2013·Memórias do Instituto Oswaldo Cruz·Euzenir Nunes SarnoHaroldo José de Matos
Sep 3, 2019·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Christian KromerTimo Buhl
Apr 17, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Annemieke GelukTom H M Ottenhoff
Sep 21, 2019·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Christian KromerTimo Buhl

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Methods Mentioned

BETA
PCR

Software Mentioned

Graph Pad Prism
STATISTICA
Microplate Manager
StatSoft

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