Peptides extracted from edible mushroom: Lentinus squarrosulus induces apoptosis in human lung cancer cells

Pharmaceutical Biology
Arisara PrateepChatchai Chaotham

Abstract

Lentinus squarrosulus Mont. (Polyporaceae) is an interesting source of diverse bioactive compounds. This is the first study of the anticancer activity and underlying mechanism of peptides extracted from Lentinus squarrosuls. Peptides were isolated from the aqueous extract of L. squarrosulus by employing solid ammonium sulphate precipitation. They were further purified by ion-exchange chromatography on diethylaminoethanol (DEAE)-cellulose and gel filtration chromatography on Sephadex G25. Anticancer activity was investigated in human lung cancer H460, H292 and H23 cells cultured with 0-40 μg/mL of peptide extracts for 24 h. Cell viability and mode of cell death were evaluated by MTT and nuclear staining assay, respectively. Western blotting was used to investigate the alteration of apoptosis-regulating proteins in lung cancer cells treated with peptide extracts (0-20 μg/mL) for 24 h. The cytotoxicity of partially-purified peptide extracts from L. squarrosulus was indicated with IC50 of ∼26.84 ± 2.84, 2.80 ± 2.14 and 18.84 ± 0.30 μg/mL in lung cancer H460, H292 and H23 cells, respectively. The extracts at 20 μg/mL induced apoptosis through the reduction of anti-apoptotic Bcl-2 protein (∼0.5-fold reduction) and up-regulation of BA...Continue Reading

References

Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Mar 3, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Roy S HerbstDavid H Johnson
Apr 3, 2004·Quality of Life Research : an International Journal of Quality of Life Aspects of Treatment, Care and Rehabilitation·Wei-Chu ChiePan-Chyr Yang
Mar 2, 2005·Nature Reviews. Cancer·J Martin Brown, Laura D Attardi
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Aug 11, 2007·International Journal of Cancer. Journal International Du Cancer·Emmanuelle WesargMartin Schuler
Oct 29, 2008·Oncogene·K W Yip, J C Reed
Dec 23, 2008·American Journal of Respiratory Cell and Molecular Biology·Tsung-Ming YangV Courtney Broaddus
Jan 24, 2009·Biochemical Pharmacology·Christian Bailly
Nov 26, 2009·The International Journal of Biochemistry & Cell Biology·Marina BagnoliDelia Mezzanzanica
Mar 27, 2010·International Journal of Cell Biology·Afshin SamaliSrinivasa M Srinivasula
Jul 31, 2010·Journal of Perinatology : Official Journal of the California Perinatal Association·W-H LimP-H Yang
Oct 26, 2010·Biochimica Et Biophysica Acta·Liam PorttMichael T Greenwood
Mar 23, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Nor Adila Mhd OmarVikineswary Sabaratnam
Apr 2, 2011·Biochimica Et Biophysica Acta·Inthrani R IndranCatherine Brenner
Oct 19, 2011·Biochemical Pharmacology·Chern Chiuh WooKwong Huat Benny Tan
Feb 22, 2012·Cancers·Ahmad R Safa, Karen E Pollok
May 1, 2012·Experimental Cell Research·Laura S DickensMarion MacFarlane
May 15, 2012·3 Biotech·Seema Patel, Arun Goyal
Jun 28, 2013·Journal of Perinatology : Official Journal of the California Perinatal Association·A A PennM P Moore
Dec 21, 2013·Nature Reviews. Molecular Cell Biology·Peter E CzabotarJerry M Adams
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Aug 25, 2015·Biochimica Et Biophysica Acta·Hassan Lemjabbar-AlaouiPetra Buchanan
Feb 3, 2016·Clinical Lung Cancer·Dariusz Adam DziedzicUNKNOWN Polish Lung Cancer Study Group (PLCSG)
Mar 23, 2017·International Journal of Medicinal Mushrooms·Aparajita Roy DasPanna Das

❮ Previous
Next ❯

Citations

Feb 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wanatchaporn ArunmaneeChatchai Chaotham
Jun 20, 2020·Applied Microbiology and Biotechnology·Vala RezvaniLeila Farahmand
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rongchen DaiHongxi Xu
Apr 22, 2021·Immunotherapy·Kimia Kardani, Azam Bolhassani

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
ion exchange chromatography
size exclusion chromatography
size-exclusion
electrophoresis
ion-exchange chromatography
gel filtration
amino acid sequencing

Software Mentioned

SPSS Statistic

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.