Peptides from the amino-terminus of RANTES cause chemotaxis of human T-lymphocytes

Biochemical and Biophysical Research Communications
T N WellsK B Bacon

Abstract

RANTES is a potent chemotactic and activating agent for a variety of leukocytes including T-lymphocytes. To identify the region of the molecule responsible for this activity, we have made overlapping 10 amino acid peptides scanning the protein. A micro-Boyden chamber chemotaxis assay showed the most efficacious peptides came from at the N-terminus. EC50 values of 8 nM (+/- 2.2 nM), 3.7 nM (+/- 2.0 nM) and 3.1 nM (+/- 2.0 nM) were calculated from dose response curves for the peptides (1-10), (3-12), and (5-14). Control peptides from other regions are not active. In THP-1 cells, none of the peptides give a Ca2+ response. The active peptides are shown to be principally chemotactic rather than chemokinetic by a checker board analysis. The results imply that the principal region of RANTES responsible for chemotaxis is located in the amino terminus.

Citations

Aug 10, 2011·Biochemical Pharmacology·Andy ChevignéSabrina Deroo
Feb 5, 2008·Vaccine·Luca VangelistaPaolo Lusso
Mar 14, 2008·Clinical and Experimental Immunology·E Mendez-EnriquezE A García-Zepeda
Jun 28, 2005·Cytokine & Growth Factor Reviews·Jean-Yves SpringaelMarc Parmentier
Aug 12, 1999·The Journal of Clinical Endocrinology and Metabolism·Y AshhabR Pujol-Borrell
Feb 1, 1996·Trends in Biotechnology·O M HowardJ J Oppenheim

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