Peptides from the scorpion Vaejovis punctatus with broad antimicrobial activity

Peptides
Santos Ramírez-CarretoErnesto Ortiz

Abstract

The antimicrobial potential of two new non-disulfide bound peptides, named VpAmp1.0 (LPFFLLSLIPSAISAIKKI, amidated) and VpAmp2.0 (FWGFLGKLAMKAVPSLIGGNKSSSK) is here reported. These are 19- and 25-aminoacid-long peptides with +2 and +4 net charges, respectively. Their sequences correspond to the predicted mature regions from longer precursors, putatively encoded by cDNAs derived from the venom glands of the Mexican scorpion Vaejovis punctatus. Both peptides were chemically synthesized and assayed against a variety of microorganisms, including pathogenic strains from clinical isolates and strains resistant to conventional antibiotics. Two shorter variants, named VpAmp1.1 (FFLLSLIPSAISAIKKI, amidated) and VpAmp2.1 (FWGFLGKLAMKAVPSLIGGNKK), were also synthesized and tested. The antimicrobial assays revealed that the four synthetic peptides effectively inhibit the growth of both Gram-positive (Staphylococcus aureus and Streptococcus agalactiaea) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria, with MICs in the range of 2.5-24.0 μM; yeasts (Candida albicans and Candida glabrata) with MICs of 3.1-50.0 μM; and two clinically isolated strains of Mycobacterium tuberculosis-including a multi-drug resistant one- wi...Continue Reading

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Citations

Dec 30, 2015·Toxins·Carlos E Santibáñez-LópezLourival D Possani
Feb 28, 2016·Toxicon : Official Journal of the International Society on Toxinology·Timoteo Olamendi-PortugalLourival D Possani
Jun 21, 2016·International Journal of Molecular Sciences·Ailton Cesar LemesKátia Flávia Fernandes
Dec 29, 2016·Biochemistry·Olapeju BolarinwaJianfeng Cai
Feb 24, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ivan ArenasGerardo Corzo
Jul 20, 2019·3 Biotech·Bency Thankappan, Jayaraman Angayarkanni
Jul 28, 2019·Marine Drugs·Santos Ramírez-CarretoClaudia Rodríguez-Almazán
Jun 27, 2019·Current Protein & Peptide Science·Sara Silva, Nuno Vale
Aug 28, 2020·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Ashish K LamiyanNeelima R Kumar
May 1, 2021·Pharmaceuticals·Gabriel S OliveiraCátia Teixeira
Jan 10, 2019·Chemical Reviews·Shantanu GuhaWilliam C Wimley

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