May 19, 1976

Peptides isolated from Enterobacter nuclease as potential polyamine binding sites

Biochimica Et Biophysica Acta
J J FrankC C Levy

Abstract

The Enterobacter nuclease, which cleaves RNA between the 3'-phosphate group of cytidylic acid and the 5'-hydroxyl group of adenylic acid, has been shown to be affected by the polyamines, spermidine, spermine and putrescine. These substances enhance the hydrolytic activity of the enzyme against both poly(C) and yeast RNA. Sperimidine and spermine also reverse the inhibition of the enzyme by the ordered polynucleotides, apparently by removing them from the surface of the enzyme. Treatment of poly(G)-bound peptides (obtained from tryptic digests of poly(G)-bound nuclease) with an excess of spermidine resulted in the isolation of spermidine-bound peptides. Purification of these peptides through ion-exchange chromatography resulted in the isolation of three spermidine-bound peptides which consisted of 17 residues (6 amino acids), 19 residues (10 amino acids), and 12 residues (9 amino acids). The binding ratio of spermidine to peptides varied from 1:1 to 3:1.

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Citations

Mentioned in this Paper

Alkalescens-Dispar Group
Amino Acids, I.V. solution additive
Polynucleotides
Putrescine
Phosphate Measurement
Digests
Plasma Protein Binding Capacity
Spermine
Alkaline Ribonuclease
Peptide Fragments

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