Peptides selected from a phage display library with an HIV-neutralizing antibody elicit antibodies to HIV gp120 in rabbits, but not to the same epitope

AIDS Research and Human Retroviruses
Royce A WilkinsonMartin Teintze

Abstract

Monoclonal antibodies specific for the conserved CD4 binding site region of the HIV envelope protein gp120 were used to select phage from two different random peptide display libraries. Synthetic peptides were made with sequences corresponding to those displayed on the selected phage, and peptide-protein fusions were expressed that contained the selected phage-displayed peptide sequence and either the N-terminal domain of the phage pIII protein or the small heat shock protein of Methanococcus jannaschii or both. For monoclonal antibody 5145A, these constructs containing the selected peptide sequences were all capable of specifically inhibiting the binding of 5145A to HIV-1 gp120. Rabbits immunized with peptide-protein fusions produced antisera that bound to recombinant HIV-1 gp120, but did not bind to HIV-infected cells nor neutralize HIV. The antisera also did not compete with CD4 or antibodies to the CD4 binding site for binding to gp120.

References

Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R BurtonR A Lerner
Jun 1, 1994·The Journal of Clinical Investigation·S H PincusM Reitz
Jan 1, 1993·Methods in Enzymology·G P Smith, J K Scott
Jun 15, 1996·Analytical Biochemistry·J B BurrittA J Jesaitis
Aug 26, 1998·Nature·K K KimS H Kim
Feb 27, 1999·Current Opinion in Biotechnology·D J Rodi, L Makowski
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·D G MyszkaM L Doyle
Feb 28, 2002·Combinatorial Chemistry & High Throughput Screening·Christopher G AddaMichael Foley
Feb 3, 2004·Journal of Receptor and Signal Transduction Research·Michael Szardenings
Jan 18, 2005·Chemical Communications : Chem Comm·Michelle L FlennikenTrevor Douglas
Feb 25, 2005·Nature·Bing ChenStephen C Harrison
Aug 31, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erez M BublilJonathan M Gershoni

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Citations

Mar 10, 2016·International Journal of Peptide Research and Therapeutics·Ewa D MicewiczPiotr Ruchala
Mar 31, 2015·Bioorganic & Medicinal Chemistry Letters·Kristine ChuaPiotr Ruchala
May 19, 2012·International Journal of Molecular Sciences·Sylvie DelhalleAndy Chevigné
Aug 3, 2019·Expert Review of Vaccines·Zachariah SternLeondios G Kostrikis

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