Peptides which bind to E-selectin and block neutrophil adhesion.

The Journal of Biological Chemistry
C L MartensC W Smith

Abstract

E-selectin is an inducible cell adhesion molecule which mediates rolling of neutrophils on the endothelium, an early event in the development of an inflammatory response. Inhibition of selectin-mediated rolling is a possible means for controlling inflammation-induced diseases, and several classes of compounds have been tested for this use. We describe here the use of recombinant peptide library screening for identification and optimization of novel ligands which bind to E-selectin. Several of these peptides bind with Kd values in the low nanomolar range and block E-selectin-mediated adhesion of neutrophils in static and flow-cell assays. Administration of the peptide to mice undergoing an acute inflammatory response reduced the extent of neutrophil transmigration to the site of inflammation, demonstrating the utility of this compound as a potential therapeutic. The identification of a peptide ligand for E-selectin suggests that the complete natural ligand for this adhesion molecule may include protein as well as carbohydrate moieties.

References

Sep 1, 1978·The American Journal of Physiology·K M BaldwinR E Lewis
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M G CullP J Schatz
May 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R H GundelL G Letts
Nov 1, 1992·The Histochemical Journal·S Hakomori
Dec 1, 1992·Current Opinion in Biotechnology·M K Robinson, P E Stephens
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J K ScottI J Goldstein
Jul 1, 1992·The Journal of Cell Biology·K L MooreR P McEver
Jul 15, 1992·Biochemical and Biophysical Research Communications·K L Moore, L F Thompson
Oct 1, 1992·The Journal of Clinical Investigation·M S MulliganP A Ward
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K R OldenburgM A Gallop
Jul 27, 1990·Science·J K Scott, G P Smith
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M TiemeyerB K Brandley
Feb 28, 1991·Nature·L J PickerE C Butcher
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M J PolleyJ C Paulson
Sep 11, 1991·Proceedings of the National Academy of Sciences of the United States of America·M I CybulskyT Collins
Oct 1, 1991·The Journal of Clinical Investigation·M S MulliganP A Ward
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D TyrrellD Asa
Aug 2, 1990·Nature·T A Springer
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S E CwirlaW J Dower
Jul 1, 1989·The Journal of Cell Biology·B R BowenL A Lasky
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M P BevilacquaM A Gimbrone
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B L LiS Pestka
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M C NeedelsM A Gallop
Dec 1, 1993·The Journal of Clinical Investigation·O AbbassiC W Smith

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Citations

Feb 22, 2002·Medicinal Research Reviews·Helena Yusuf-MakagiansarTeruna J Siahaan
Jan 1, 1996·International Journal of Clinical & Laboratory Research·G BertonC A Lowell
May 15, 2009·European Radiology·Caroline WyssXavier Montet
Apr 25, 1997·Journal of Immunological Methods·C R BertozziS D Rosen
Apr 12, 2002·Trends in Biotechnology·Behjatolah Monzavi-KarbassiThomas Kieber-Emmons
Jun 27, 2002·Peptides·Insug OMagdalena Blaszczyk-Thurin
Dec 12, 2012·Analytical Chemistry·Lucía VanrellGualberto Gonzalez-Sapienza
Apr 9, 2008·Bioconjugate Chemistry·A González-TecheraG González-Sapienza
Feb 14, 2002·Chemical Reviews·Joseph J Lundquist, Eric J Toone
Sep 26, 2012·Nature Reviews. Rheumatology·James M MountzJohn D Mountz
Dec 11, 2002·Journal of Neurochemistry·Maryline Simon-HaldiMelitta Schachner
Sep 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bettina SehnertReinhard Edmund Voll
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·D O'ConnellA Varki
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·S D YanofskyR W Barrett
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·P Sears, C H Wong
May 29, 2002·Hybridoma and Hybridomics·Magdalena Thurin, Thomas Kieber-Emmons
Sep 22, 1998·Antisense & Nucleic Acid Drug Development·R D JenisonD Parma
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·H B Lowman
Oct 6, 2012·Biological & Pharmaceutical Bulletin·Michiko N FukudaKazuhiro Sugihara
Feb 17, 2001·Biochemical and Biophysical Research Communications·S JeongJ Yu
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·S L HarrisJ K Scott
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Chikara OhyamaMinoru Fukuda
Oct 26, 2005·Neoplasia : an International Journal for Oncology Research·Martin FunovicsLee Josephson
Jul 28, 2004·The American Journal of Pathology·Qing Li, Bjorn R Olsen
Dec 15, 2010·Ultrasound in Medicine & Biology·Astrid Hilde MyrsetRoald Skurtveit
Sep 4, 2009·Molecular Immunology·Victor G SendraFernando J Irazoqui
Aug 25, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yosi ShamayAyelet David

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