Peptides with more than one 106-amino acid sequence motif are needed to mimic the structural stability of spectrin.

The Journal of Biological Chemistry
N MenhartL W Fung

Abstract

The primary sequence of human erythrocyte spectrin contains repetitive homologous sequence motifs of approximately 106 amino acids with 22 such motifs in the alpha-subunit and 17 in the beta-subunit. These homologous sequence motifs have been proposed to form domains with a triple-helical bundle type structure (Speicher, D. W., and Marchesi, V. T. (1984) Nature 311, 177-180; Parry, D. A. D., Dixon, T. W., and Cohen, C. (1992) Biophys. J. 61, 858-867). In this study, we show that these sequence motifs, while they do form compact proteolytically resistant units, are not completely independent. Peptides composed of two or three such motifs in tandem are substantially more stable than peptides composed of a single motif, as measured by proteolysis or by fluorescence or circular dichroism studies of urea or thermal denaturation. Circular dichroism and infrared spectroscopy measurements also indicate that these larger, more stable peptides exhibit greater secondary structure. In these respects, the peptides with tandem sequence motifs are more similar to intact spectrin than the peptide with a single sequence motif. Thus, we conclude that peptides with more than one sequence motif model spectrin more adequately than the peptides with...Continue Reading

References

Jul 25, 1979·Biochimica Et Biophysica Acta·G B Ralston, J C Dunbar
Jan 1, 1978·Journal of Supramolecular Structure·G B Ralston
Apr 14, 1992·Biochemistry·D M BudzynskiL W Fung
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E WinogradD Branton
Jun 1, 1980·European Journal of Biochemistry·R CalvertW Gratzer
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·N Mohandas, E Evans
Oct 1, 1994·European Journal of Biochemistry·A P GilmoreD R Critchley
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·R I MacDonaldM Saraste
Nov 1, 1993·Protein Science : a Publication of the Protein Society·C A RoyerC R Matthews
Jan 1, 1993·Annual Review of Cell Biology·V Bennett, D M Gilligan
Jan 28, 1994·Journal of Molecular Biology·E KahanaW B Gratzer
Dec 1, 1995·Protein Science : a Publication of the Protein Society·M J BennettD Eisenberg
Apr 23, 1996·Biochemistry·G RalstonD Branton

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Citations

Apr 28, 2000·European Journal of Biochemistry·L GalluzziM C Lecomte
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May 18, 2005·Journal of Molecular Biology·Vanessa OrtizDennis E Discher
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