Peptidoglycan recognition protein S2 from silkworm integument: characterization, microbe-induced expression, and involvement in the immune-deficiency pathway

Journal of Insect Science
Jie YangJinmei Wu

Abstract

Peptidoglycan recognition protein (PGRP) binds specifically to peptidoglycan and plays an important role as a pattern recognition receptor in the innate immunity of insects. The cDNA of a short-type PGRP, an open reading frame of 588 bp encoding a polypeptide of 196 amino acids, was cloned from Bombyx mori. A phylogenetic tree was constructed, and the results showed that BmPGRP-S2 was most similar to Drosophila melanogaster PGRP (DmPGRP-SA). The induced expression profile of BmPGRP-S2 in healthy Escherichia coli- and Bacillus subtilis-challenged B. mori was measured using semiquantitative reverse transcriptase polymerase chain reaction analysis. The expression of BmPGRP-S2 was upregulated at 24 h by E. coli and Ba. subtilis challenge. In addition, in the integument of B. mori, RNAi knockdown of BmPGRP-S2 caused an obvious reduction in the transcription expression of the transcription factor Relish and in antibacterial effector genes Attacin, Gloverin, and Moricin. The results indicated that BmPGRP-S2 participates in the signal transduction pathway of B. mori.

References

Sep 1, 1995·The Biochemical Journal·S Hara, M Yamakawa
Feb 1, 1995·Current Opinion in Immunology·J A Hoffmann
Dec 15, 1995·The Journal of Biological Chemistry·S Hara, M Yamakawa
Jun 7, 1996·The Journal of Biological Chemistry·H YoshidaM Ashida
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·M S DushayD Hultmark
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·B LemaitreJ A Hoffmann
Jul 30, 1999·Developmental and Comparative Immunology·P BuletD Hoffmann
Nov 9, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·L Otvos
Dec 14, 2001·Nature·T Kaisho, S Akira
Apr 16, 2002·Science·Ranjiv S KhushBruno Lemaitre
Sep 13, 2002·Insect Biochemistry and Molecular Biology·M D Lavine, M R Strand
Dec 24, 2002·The Journal of Biological Chemistry·Peter MellrothHakan Steiner
Jun 25, 2003·Biochemical and Biophysical Research Communications·Eva GeliusHåkan Steiner
Sep 25, 2003·The Journal of Biological Chemistry·Zheng-Ming WangRoman Dziarski
May 14, 2004·The Journal of Biological Chemistry·Rongjin GuanRoy A Mariuzza
Jun 18, 2004·Immunological Reviews·Håkan Steiner
Jan 11, 2005·Protein and Peptide Letters·Philippe Bulet, Reto Stöcklin
Jan 18, 2005·Current Opinion in Immunology·Julien RoyetJules A Hoffmann
Aug 26, 2006·Genome Biology·Roman Dziarski, Dipika Gupta
Oct 6, 2006·Applied and Environmental Microbiology·Caroline AnselmeAbdelaziz Heddi
Jan 5, 2007·Annual Review of Immunology·Bruno Lemaitre, Jules Hoffmann
Sep 11, 2007·Bioscience, Biotechnology, and Biochemistry·Yoichi KanekoMinoru Yamakawa
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew F Rowley, Adam Powell
Dec 14, 2007·Archives of Insect Biochemistry and Physiology·Shinpei KawaokaToru Shimada
Jun 6, 2008·The Journal of Biological Chemistry·Nirotpal Mrinal, Javaregowda Nagaraju
Oct 7, 2008·Insect Biochemistry and Molecular Biology·Hiromitsu TanakaMinoru Yamakawa
Apr 7, 2009·Microbes and Infection·Bernard CharrouxJulien Royet
Dec 8, 2009·Bioscience, Biotechnology, and Biochemistry·Seiichi FurukawaMinoru Yamakawa
Feb 1, 2008·Insect Science·Shubha Govind
May 5, 2011·World Journal of Biological Chemistry·Jing-Huan WangMika Rämet
Jan 13, 2012·Journal of Innate Immunity·Monica M Davis, Ylva Engström

❮ Previous
Next ❯

Citations

Mar 19, 2016·Archives of Insect Biochemistry and Physiology·Hiromitsu Tanaka, Aki Sagisaka
Oct 12, 2019·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Qiang WangKeping Chen
Jan 12, 2021·Archives of Insect Biochemistry and Physiology·Fang-Fang LiuXiang-Jun Rao
Oct 9, 2019·International Journal of Biological Macromolecules·Zhantao ZhangXiaoxia Xu
Mar 2, 2021·Frontiers in Microbiology·Jizhen WeiShiheng An
Jan 1, 2018·Developmental and Comparative Immunology·Kangkang Chen, Zhiqiang Lu

❮ Previous
Next ❯

Datasets Mentioned

BETA
KF906541

Methods Mentioned

BETA
Reverse Transcription-Polymerase Chain Reaction
PCR
electrophoresis

Software Mentioned

Mega
ClustalW
TMHMM
ClustalX1
Basic Local Alignment Search Tool ( BLAST )
MEGA4

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.