PMID: 241742Nov 1, 1975

Peptidoglycan synthesis in L-phase variants of Bacillus licheniformis and Bacillus subtilis

Journal of Bacteriology
J B Ward

Abstract

Stable L-phase variants isolated from Bacillus licheniformis and Bacillus subtilis, when grown in osmotically stabilized media, do not synthesize peptidoglycan but have been found to accumulate the nucleotide precursors of this polymer. The enzymes involved in the synthesis of these precursors and the later membrane-bound stages of peptidoglycan synthesis have been investigated, and the L-phase variants have been shown to contain lesions, which provide a rational explanation for the absence of peptidoglycan and for the nature of the precursor accumulated. The majority of the L-phase variants contained a single enzymic defect, but two strains were isolated with double lesions. Five out of seven strains examined accumulated uridine 5'-diphosphate (UDP)-MurAc-L-ala-D-glu and were unable to synthesize diaminopimelic acid as a consequence of a defect in aspartate-beta-semialdehyde dehydrogenase activity. Two strains were deficient in UDP-MurAc: L-alanine ligase and accumulated UDP-MurAc. One strain accumulated the complete nucleotide precursor UDP-MurAc-L-ala-D-glu-mA2pm-D-ala-D-ala and was deficient in phospho-N-acetylmuramyl pentapeptide translocase. A second strain also had this lesion, together with defective aspartate-beta-semi...Continue Reading

Citations

Sep 12, 2012·Antimicrobial Agents and Chemotherapy·Diana WolfThorsten Mascher
Aug 1, 1978·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·P Virkola
Feb 13, 2009·Nature·M LeaverJ Errington

Related Concepts

Abufne
Transaminases
Aspartate Kinase III
Aspartate-Semialdehyde Dehydrogenase
Natto Bacteria
Carboxypeptidase
Plasma Membrane
Cell Wall
Cell-Free System
Diaminopimelic Acid

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