Peptidomic strategy for purification and identification of potential ACE-inhibitory and antioxidant peptides in Tetradesmus obliquus microalgae

Analytical and Bioanalytical Chemistry
Carmela Maria MontoneAldo Laganà

Abstract

Microalgae are unicellular marine organisms that have promoted complex biochemical pathways to survive in greatly competitive marine environments. They could contain significant amounts of high-quality proteins which, because of their structural diversity, contain a range of yet undiscovered novel bioactive peptides. In this work, a peptidomic platform was developed for the separation and identification of bioactive peptides in protein hydrolysates. In this work, a peptidomic platform was developed for the extraction, separation, and identification of bioactive peptides in protein hydrolysates. Indeed, extraction of proteins from recalcitrant tissues is still a challenge due to their strong cell walls and high levels of non-protein interfering compounds. Therefore, seven different protein extraction protocols, based on mechanical and chemical methods, were tested in order to produce high-quality protein extracts. Proteins obtained by means of the best protocol, consisting of milling the recalcitrant tissue with glass beads, were subjected to enzymatic digestion with Alcalase® and subsequently the hydrolysate was purified by two-dimensional semi-preparative reversed phase liquid chromatography. Fractions were assayed for antioxi...Continue Reading

References

Jun 25, 1999·Free Radical Biology & Medicine·R ReC Rice-Evans
Feb 19, 2008·Journal of Lipid Research·Vitali MatyashDominik Schwudke
May 25, 2010·Bioresource Technology·Cynthia Victoria González LópezJosé María Fernández Sevilla
Sep 9, 2011·Folia Microbiologica·Magdalena Klimek-OchabPaweł Kafarski
Sep 4, 2012·Bioresource Technology·Gursong YooJi-Won Yang
Mar 22, 2014·Journal of the Science of Food and Agriculture·Zhipeng YuJingbo Liu
Sep 17, 2014·Microbial Biotechnology·Sara P Cuellar-BermudezRoberto Parra-Saldivar
Feb 11, 2015·Journal of Proteomics·Susy PiovesanaAldo Laganà
Jan 24, 2016·Analytical and Bioanalytical Chemistry·Anna Laura CapriottiAldo Laganà
May 2, 2016·Bioresource Technology·Jessica Hartwig DuarteJorge Alberto Vieira Costa
Nov 7, 2016·Journal of Biotechnology·Celma Estefanía Ochoa-MéndezRuth Elena Soria-Guerra
Sep 7, 2017·Food Research International·Pádraigín A HarnedyRichard J FitzGerald
Jan 22, 2018·Analytical and Bioanalytical Chemistry·Susy PiovesanaAldo Laganà

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Citations

Oct 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carmela Maria MontoneAldo Laganà
Oct 31, 2019·Journal of Food Biochemistry·Zhipeng ZouRong He
May 29, 2020·Critical Reviews in Food Science and Nutrition·Matheus Lopes AmorimMarcio Arêdes Martins
May 8, 2020·Antibiotics·Reuben MaghembeRajni Hatti-Kaul
Nov 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chiara CavaliereAldo Laganà
Oct 31, 2020·Critical Reviews in Food Science and Nutrition·Seyed Fakhreddin HosseiniDavid Julian McClements
Oct 29, 2020·Oxidative Medicine and Cellular Longevity·Li ZhangLingmei Qian
Dec 16, 2020·Marine Drugs·Vida ŠimatFatih Özogul
Mar 23, 2021·Frontiers in Pharmacology·Umme Tamanna Ferdous, Zetty Norhana Balia Yusof
Sep 9, 2019·Analytica Chimica Acta·Lidia Montero, Miguel Herrero
Apr 26, 2020·Food Research International·Julia BechauxVéronique Santé-Lhoutellier
Nov 12, 2021·Applied Biochemistry and Biotechnology·Davoodbasha MubarakAliNavabshan Irfan

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