Peptidomics in Drosophila melanogaster

Briefings in Functional Genomics & Proteomics
Liliane Schoofs, Geert Baggerman

Abstract

In analogy with proteomics technology, where all proteins expressed in a cell or tissue are analysed, the peptidomic approach aims at the simultaneous visualisation and identification of the whole peptidome of a cell or tissue, ie all expressed peptides with their post-translational modifications. With nanoscale liquid chromatography (nanoLC), combined with mass spectrometry and subsequent database searching, the peptidome of the Drosophila larval brain has been identified at the amino acid sequence level. In a single experiment involving only 50 Drosophila larval brains, one can obtain a display of the expressed peptides. In this paper, current peptidomics technology will be explained, using Drosophila as an example. Compared with the 400,000 Drosophila whole bodies that were required as a starting material for traditional biochemical peptide purification rounds, the authors are convinced that peptidomics technology, which in the future will certainly be applied to the analysis of different physiological states, has the inherent potential to bring about a true revolution in the study of the molecular physiology of Drosophila.

Citations

Dec 17, 2009·Journal of Proteome Research·Gerben MenschaertWim Van Criekinge
Jan 31, 2009·Proceedings of the National Academy of Sciences of the United States of America·Axel BrockmannJonathan V Sweedler
Nov 29, 2011·Genome Biology·Emmanuel LadoukakisJuan Pablo Couso
Sep 30, 2009·Insect Biochemistry and Molecular Biology·Susan E BrownPeter D East
Aug 10, 2007·General and Comparative Endocrinology·Ank ReumerLiliane Schoofs
Dec 12, 2007·Plant Biotechnology Journal·Naser FarrokhiJudy A Brusslan
Jun 29, 2010·Journal of Proteomics·Alexey Veraksa
Apr 6, 2013·General and Comparative Endocrinology·Shireen-A DaviesJulian A T Dow
Aug 11, 2004·Developmental Biology·Peter VerleyenLiliane Schoofs
Jan 27, 2018·Journal of the American Society for Mass Spectrometry·Max DiesnerSusanne Neupert
Jan 13, 2006·Mass Spectrometry Reviews·Lloyd D FrickerFa-Yun Che
Apr 13, 2006·Expert Review of Proteomics·Wannes D'HertogLut Overbergh
Dec 24, 2018·Expert Review of Proteomics·Samantha Louise EdwardsLiliane Schoofs
Nov 6, 2007·Journal of Proteome Research·Fa-Yun CheLloyd D Fricker
Jul 25, 2007·Chemical Reviews·Gert Lubec, Leila Afjehi-Sadat

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