PMID: 1144067Jun 1, 1975

Peptidyl - tRNA hydrolase and RNase activities in cell fractions of rat liver used in in vitro reconstitution of rough membrane

Nucleic Acids Research
A A HochbergE Ziv

Abstract

Peptidyl-tRNA hydrolase and RNase activities have been studied in those fractions of rat liver, which are used in in vitro reconstitution of rough membrane, because these enzymes may interfere with the in vitro reconstitution. It was found that smooth membrane has an active peptidyl-tRNA hydrolase, while the other fractions tested, polyribosomes, rough membrane, stripped rough membrane and the post-microsomal supernatant had no, or very low, peptidyl-tRNA hydrolase activity. Polyribosomes, rough and stripped rough membrane have RNase activity; this activity could be completely inhibited by rat liver RNase inhibitor. It is shown that RNase inhibitor is an obligatory component in in vitro experiments, in which rough membrane is reconstituted from stripped rough membrane, ribosomes and mRNA.

References

Sep 25, 1974·Journal of Molecular Biology·N BorgeseD D Sabatini
Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·F CuzinF Chapeville
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·Z VogelD Elson
Jan 1, 1969·Biochimica Et Biophysica Acta·N De GrootY Lapidot
Jul 7, 1971·Nature: New Biology·C BaglioniM Zauderer
Mar 1, 1969·Archives of Biochemistry and Biophysics·A A GribnauH Bloemendal
Jan 1, 1955·The Journal of Biophysical and Biochemical Cytology·G Palade

Citations

Mar 31, 1982·Molecular Biology Reports·H LorberboumA A Hochberg

Related Concepts

Metazoa
Esterases
Glycine, Monopotasssium Salt
Liver
Tissue Membrane
Polypeptides
Endorphenyl
Polyribosomes
Alkaline Ribonuclease
Ribosomes

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