PMID: 1144067Jun 1, 1975

Peptidyl - tRNA hydrolase and RNase activities in cell fractions of rat liver used in in vitro reconstitution of rough membrane

Nucleic Acids Research
A A HochbergE Ziv


Peptidyl-tRNA hydrolase and RNase activities have been studied in those fractions of rat liver, which are used in in vitro reconstitution of rough membrane, because these enzymes may interfere with the in vitro reconstitution. It was found that smooth membrane has an active peptidyl-tRNA hydrolase, while the other fractions tested, polyribosomes, rough membrane, stripped rough membrane and the post-microsomal supernatant had no, or very low, peptidyl-tRNA hydrolase activity. Polyribosomes, rough and stripped rough membrane have RNase activity; this activity could be completely inhibited by rat liver RNase inhibitor. It is shown that RNase inhibitor is an obligatory component in in vitro experiments, in which rough membrane is reconstituted from stripped rough membrane, ribosomes and mRNA.


Sep 25, 1974·Journal of Molecular Biology·N BorgeseD D Sabatini
Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·F CuzinF Chapeville
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·Z VogelD Elson
Jan 1, 1969·Biochimica Et Biophysica Acta·N De GrootY Lapidot
Jul 7, 1971·Nature: New Biology·C BaglioniM Zauderer
Mar 1, 1969·Archives of Biochemistry and Biophysics·A A GribnauH Bloemendal
Jan 1, 1955·The Journal of Biophysical and Biochemical Cytology·G Palade


Mar 31, 1982·Molecular Biology Reports·H LorberboumA A Hochberg

Related Concepts

Glycine, Monopotasssium Salt
Tissue Membrane
Alkaline Ribonuclease

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Related Papers

Metabolism: Clinical and Experimental
R L Greif, E F Eich
Biochemical and Biophysical Research Communications
R M Kershner, W L Meyer
Biologia Neonatorum. Neo-natal Studies
D P Alexander, D A Nixon
© 2021 Meta ULC. All rights reserved