PMID: 36674Dec 1, 1978

Peptones and mycological reproducibility

Sabouraudia
F C OddsA B Abbott

Abstract

The pH reactions, ultraviolet spectra and phosphorus content of solutions of a variety of commercially available peptones all indicated, predictably, considerable differences in the chemical composition of the peptones. The effects of these differences on the outcome of experiments with Candida albicans grown in different peptone media was investigated. The fungus produced germ tubes equally effectively on all such media provided that the inoculum was kept to 10(6) blastospores/ml or less. However, expression of inducible enzyme activities in C. albicans varied extensively from peptone to peptone; there was, for example, an inverse relationship between the inorganic phosphorus content of peptones and the amount of acid phosphomonoesterase detectable in intact blastospores. The results indicated that use of different peptones in "Sabouraud's" media by different laboratories may account for some, but not all, published instances of irreproducibility of experiments with C. albicans.

References

Dec 22, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·P H FernanadoL P Samaranayake
Jun 6, 2006·Applied and Environmental Microbiology·Kenneth W NickersonJacob M Hornby
Mar 10, 2004·Applied and Environmental Microbiology·Jacob M HornbyKenneth W Nickerson
Apr 1, 1988·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·M J Kennedy, R L Sandin
Jan 1, 1991·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·F C Odds

Citations

Mar 1, 1976·Infection and Immunity·N DabrowaD H Howard
Jan 1, 1976·Infection·M Majer
Mar 1, 1975·Canadian Journal of Microbiology·E G EvansK T Holland
Dec 1, 1973·American Journal of Clinical Pathology·K R JoshiD N Parr
Jul 1, 1971·Canadian Journal of Microbiology·D N MardonE Balish
Jun 1, 1971·American Journal of Clinical Pathology·C T Dolan, D M Ihrke
Jun 1, 1969·Sabouraudia·S Bernander, L Edebo
Nov 1, 1962·Journal of Clinical Pathology·D W Mackenzie

Related Concepts

Acid Phosphatase
Candida albicans
Cyclodictyon albicans
Daiotyla albicans
Cineraria albicans
Carex albicans
Codia albicans
Peptones
Phosphate Measurement
Glucose, (beta-D)-Isomer

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