Abstract
Olive oil and fish oils are known to possess beneficial properties for human health. We investigated whether different oils and fatty acids alone were able to decrease oxidative stress induced on corneal cells. In our in vivo study, rats were fed with marine oils rich in polyunsaturated fatty acids (PUFA) or refined olive oil during 28 days. At the end of the protocol, corneas were analysed for their fatty acids composition to study the incorporation of fatty acids in cell membranes. In our in vitro study, a human corneal cell line was incubated with marine oils or refined olive oil and subjected to oxidative stress (tBHP 50 muM, 1 hour). Effects on reactive oxygen species generation, mitochondria and caveolin-1 expression were studied using microcytofluorometry, flow cytometry and confocal microscopy. Our results indicate that dietary oils changed the fatty acids composition of corneal cell membranes. According to our results, PUFA-rich oils and refined olive oil (free of antioxidants) blocked reactive oxygen species production. Oleic acid, the major fatty acid of olive oil, also decreased oxidative stress. Moreover, oleic acid modified caveolin-1 expression. Antioxidant properties of oleic acid could be due to disruption of m...Continue Reading
References
Jan 24, 1992·Science·R G AndersonS W Lacey
Nov 1, 1990·Pediatric Research·R D UauyD R Hoffman
Jun 30, 1995·The Journal of Biological Chemistry·S LiM P Lisanti
Nov 15, 1996·The Journal of Biological Chemistry·B RoyA S Lee
Nov 15, 1996·The Journal of Biological Chemistry·S LiM P Lisanti
Nov 5, 1997·The Journal of Biological Chemistry·G García-CardeñaW C Sessa
Dec 24, 1997·Biological Trace Element Research·H M MeltzerT A Ydersbond
Aug 19, 1999·European Journal of Clinical Nutrition·A JenkinsonG G Duthie
Dec 5, 2000·Frontiers in Bioscience : a Journal and Virtual Library·A SchlegelM P Lisanti
Oct 31, 2001·Histochemistry and Cell Biology·C Mineo, R G Anderson
Nov 24, 2001·Trends in Cell Biology·M Edidin
Dec 18, 2001·Medicinal Research Reviews·Francesco VisioliClaudio Gall
Mar 29, 2002·The Journal of Investigative Dermatology·Robert GniadeckiHans Christian Wulf
Jul 23, 2002·Molecular Biology of the Cell·Daniela VolonteFerruccio Galbiati
Nov 24, 2004·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·R W OwenH Bartsch
Feb 4, 2005·Molecular Biology of the Cell·Albert PolRobert G Parton
Oct 12, 2005·Biochemical Pharmacology·Kesheng ZhaoHazel H Szeto
Dec 15, 2005·Biochimica Et Biophysica Acta·Shey-Shing SheuM W Anders
Oct 27, 2007·Investigative Ophthalmology & Visual Science·Toihiri SaidPatrice Rat
Jan 29, 2008·Recent Patents on Cardiovascular Drug Discovery·Gianluca ColussiLeonardo A Sechi
Citations
Jun 18, 2011·Nutrition & Metabolism·Mélody DutotPatrice Rat
Sep 26, 2015·PloS One·Teddy LéguillierPatrice Rat
Oct 16, 2015·Applied Biochemistry and Biotechnology·Anaïs WakxPatrice Rat
Apr 15, 2017·Chemistry and Physics of Lipids·Meryam DebbabiGérard Lizard