Percoll-purified and photosynthetically active chloroplasts from Arabidopsis thaliana leaves

Plant Physiology and Biochemistry : PPB
Daphné Seigneurin-BernyNorbert Rolland

Abstract

The availability of the complete genome sequence of Arabidopsis thaliana and of large collections of insertion mutants paved the way for systematic studies of gene functions in this organism, thus requiring adapting biochemical and physiological tools to this model plant. For physiological analysis of photosynthesis, methods combining high level of chloroplast purity and preservation of the photosynthetic activity were missing. Here, we describe a rapid method (less than 1h) to obtain Percoll-purified and photosynthetically active chloroplasts from Arabidopsis leaves retaining almost 90% of the Vmax of photosynthesis measured in the starting leaves from plants grown under a light intensity of 150mumolphotonm(-2)s(-1) and 80% of their initial photosynthetic rate after 3h of storage.

References

Mar 10, 1969·Biochemical and Biophysical Research Communications·M D Hatch, C R Slack
Oct 15, 1984·The Biochemical Journal·Z G Cerović, M Plesnicar
Aug 8, 2001·The Plant Journal : for Cell and Molecular Biology·L M Fitzpatrick, K Keegstra
Oct 10, 2002·FEBS Letters·Henrik Aronsson, Paul Jarvis
May 27, 2003·Molecular & Cellular Proteomics : MCP·Myriam FerroNorbert Rolland
Jul 31, 2003·The Plant Journal : for Cell and Molecular Biology·Petra RennéAndreas P M Weber
Sep 30, 1961·Biochimica Et Biophysica Acta·J BRUINSMA
Apr 22, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Alexander SchulzAlexandra Mant

❮ Previous
Next ❯

Citations

Sep 17, 2011·Plant Molecular Biology·Piotr KolesińskiAndrzej Szczepaniak
Oct 4, 2011·BMC Plant Biology·Erika Kovács-BogdánBettina Bölter
Aug 2, 2012·BMC Plant Biology·Madlen SchillerIris Steinebrunner
Feb 11, 2014·Journal of Experimental Botany·Sylvain BoutignyDaphné Seigneurin-Berny
Dec 5, 2013·The Plant Journal : for Cell and Molecular Biology·Guangyong LiJames R Alfano
Nov 18, 2009·Analytical Biochemistry·Jeannette KleyWilhelm Boland
May 25, 2016·Nature Communications·Andrei HerdeanBjörn Lundin
Jan 1, 2013·Plants·Agostinho Gomes Rocha, Ute C Vothknecht
Oct 3, 2018·Applications in Plant Sciences·Najmeh NasiriHamid Najafi-Zarrini
Oct 14, 2018·International Journal of Molecular Sciences·Vladimír SkalickýOndřej Novák
May 14, 2021·Frontiers in Plant Science·Jinjie AnHongbo Gao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.