Perfect wound healing in the keratin 8 deficient mouse embryo

Cell Motility and the Cytoskeleton
J BrockP Martin

Abstract

It is generally believed that the strength and structural integrity of both adult and embryonic epithelia comes, at least in part, from their internal cytoskeletal network of keratin filaments and associated cell:cell junctions. Indeed, recent keratin depletion experiments in Xenopus suggest that the capacity of embryonic epithelia to undergo natural morphogenetic movements such as gastrulation, or artificially triggered epithelial movements such as wound closure, are severely compromised in the absence of the predominant embryonic keratin, K8 [Torpey et al., 1992: Nature 357:413-415; Klymkowsky et al., 1992: Proc. Natl. Acad. Sci. USA 89:8736-8740]. These experiments contrast with studies of genetically K8 deficient mouse embryos which undergo gastrulation quite normally and, dependent upon background strain, can survive until beyond birth [Baribault et al., 1993: Genes Dev. 7:1191-1202; Baribault et al., 1994: Genes Dev. 8:2964-2973], but to date no wound healing investigations have been carried out on mK8-mice. In this article, we report our studies of healing in embryonic day 11.5 mouse embryos, wounded by amputation of the hindlimb bud and then cultured in roller bottles. In wild-type embryos, wound closure puts severe str...Continue Reading

References

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Citations

Feb 22, 2012·Cellular and Molecular Life Sciences : CMLS·Maria Teresa Abreu-BlancoSusan M Parkhurst
Aug 1, 2009·Journal of Dermatological Science·Laurie G HudsonDonna F Kusewitt
Aug 20, 2010·Molecular Carcinogenesis·Stephanie H ShirleyDonna F Kusewitt
Dec 1, 1996·Cancer Metastasis Reviews·R G OshimaC Caulín
Sep 5, 2002·Cell Motility and the Cytoskeleton·Lance A DavidsonRay Keller
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D M ToivolaJ E Eriksson
Jan 6, 1999·Archives of Pharmacal Research·W J KimM T Longaker
Jan 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Stacy MazzalupoPierre A Coulombe
Apr 8, 2020·Precision Clinical Medicine·Wengeng ZhangMarianna Bei

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