Perfluorooctanoic acid disrupts the blood-testis barrier and activates the TNFα/p38 MAPK signaling pathway in vivo and in vitro

Archives of Toxicology
Yin LuJiayin Dai

Abstract

Perfluorooctanoic acid (PFOA) is correlated with male reproductive dysfunction in animals and humans, but the underlying mechanisms for this remain unknown. To explore the potential reproductive toxicity of PFOA, we studied blood-testis barrier (BTB) damage using in vivo and in vitro models. Male mice were gavage-administered PFOA (0-20 mg/kg/d) for 28 consecutive days, and breeding capacity and permeability of the Sertoli cell-based BTB were estimated. Primary Sertoli cells (SCs) were exposed to PFOA (0-500 μM) for 48 h, and transepithelial electrical resistance (TER) was assessed. Furthermore, BTB-associated protein expression, TNFα content, and phosphorylation and protein levels of the mitogen-activated protein kinase (MAPK) pathway were detected. An apparent decrease in mated and pregnant females per male mouse as well as litter weight was observed. Marked BTB damage was evidenced by increased red biotin fluorescence in the lumen tubular of the testes and the decrease in TER in SCs in vitro. The protein levels of claudin-11, connexin-43, N-cadherin, β-catenin, and occludin were significantly decreased in the testes and also in the SCs in vitro except for N-cadherin and β-catenin. TNFα content showed a dose-dependent increas...Continue Reading

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Citations

Nov 11, 2016·Archives of Toxicology·Taiki AbeKouichi Yoshinari
Sep 30, 2020·Biological Trace Element Research·Tianjiao LuShuxia Guo
Mar 18, 2020·International Journal of Molecular Sciences·Andressa GonsioroskiJodi A Flaws
Oct 5, 2019·Oxidative Medicine and Cellular Longevity·Jifeng YeQingbo Guan
Oct 12, 2017·Frontiers in Pharmacology·Qianying LiuZonghui Yuan
Aug 17, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Bo MaQi Zhang
Nov 2, 2020·Journal of Hazardous Materials·Sayed Esmaeil MousaviLode Godderis
Jan 20, 2021·Chemosphere·Jialu BaoXiaodan Wang
Oct 22, 2020·Chemosphere·Zhongyuan WangZengjun Wang
Mar 30, 2021·Frontiers in Endocrinology·Agostina GorgaSilvina Beatriz Meroni
May 1, 2021·International Journal of Environmental Research and Public Health·Pheruza Tarapore, Bin Ouyang
Mar 3, 2020·Environmental Science & Technology·Hin Ting WanChris Kong Chu Wong
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Bo ZhangShuangbao Gun

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